FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0120_pe_1.3510000004676e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0120_pe_1.3510000004676e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11338
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGCTCTGGATCTCGTATGCCGTC253122.323161051331805No Hit
TCTTATACACATCTCCGAGCCCACGAGACAGCTCTGGATCTCGTATGCCGT1491.314164755688834No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAGCTCTGGATCTCGTATGCCG660.5821132474863292No Hit
TTATACACATCTCCGAGCCCACGAGACAGCTCTGGATCTCGTATGCCGTCT540.47627447521608746No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC2250.043.045
ATGCCGT2350.041.1702144
GTATGCC2400.040.31250442
TATGCCG2400.040.31250443
CGTATGC2350.040.21276541
TCGTATG2350.040.21276540
CAGCTCT2450.039.48979628
TCTGGAT2450.039.48979632
ACGAGAC2450.039.48979622
CTGGATC2450.039.48979633
TGGATCT2450.039.48979634
CGAGACA2450.039.48979623
TCCGAGC2450.039.48979613
ACAGCTC2450.039.48979627
ATCTCGT2400.039.37500437
GGATCTC2400.039.37500435
TCTCGTA2400.039.37500438
GATCTCG2400.039.37500436
CTCGTAT2400.039.37500439
AGCTCTG2500.038.729
Produced by FastQC (version 0.11.2)