FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0120_pd_96.35100000046745.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0120_pd_96.35100000046745.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences123
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCGTC32.4390243902439024No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAAC32.4390243902439024No Hit
GACCTCAACACGATCCTGTCCACACGCGACCACCGGCTGAACGTGCATCAC10.8130081300813009No Hit
GTCTTTCCCATTTCTTTTTGAAGTCTTTTAACAAGTTTTTGAAGCTGTCTC10.8130081300813009No Hit
CATAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAGTACAA10.8130081300813009RNA PCR Primer, Index 44 (96% over 30bp)
CTTATACACATCTCCGAGCCCACGAGACCCTGGCCCATCTCGTATGCCGTC10.8130081300813009No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAAGTACTCTGCGT10.8130081300813009No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACTGTCTCTTATACACATC10.8130081300813009No Hit
GCCTTTGCAACATCAGTGCAATACTGTGAAGACCTTCCTTCCCTGGGATCG10.8130081300813009No Hit
AACCATGACTTTGGGACCTTAGCTGGCGGTCTGGGTTGTTTCCCTCTTCAC10.8130081300813009No Hit
CCTTACGGGTTTACTCACCCGTTGACTCGCACACATGTCAGACTCCTTGGT10.8130081300813009No Hit
GTGCAATTCCAATTCAAAACTGGTAAGGTCACAAATTGAATCAAGGAAATG10.8130081300813009No Hit
CCCCCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTC10.8130081300813009No Hit
AGCTCACAGCATGTGCATTTTTGTGTACGGGGCTGTCACCCTGTATCGCGC10.8130081300813009No Hit
GCGTCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCC10.8130081300813009No Hit
CTGTGCCGTCAGACAGATCAGCCGGATCGATAGTGCCCGGGCGTCCCACCT10.8130081300813009No Hit
TATTTATCCTCTTCCTCATTTTGTTGATACCAAATGGATTACTCTTGCTCC10.8130081300813009No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA10.8130081300813009No Hit
AACGCAGAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGACTGTCTCTT10.8130081300813009No Hit
CATAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAACTGAAGAGCG10.8130081300813009RNA PCR Primer, Index 44 (96% over 30bp)
GGCGATGAAGGACGTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAA10.8130081300813009No Hit
ACTGAGTTCTTTGTTTTCTCCTCAAATAGAATGGGGCTGTCTCTTATACAC10.8130081300813009No Hit
CTGCCCGTACTCTGCGTTGATACCACCCAAAAAAAAAAAAAAAAAAAAAAA10.8130081300813009No Hit
GCCTTATGGGACATCTACTGTAGAAGATATCCAGAGAGATGTAGTGGCCAC10.8130081300813009No Hit
CTTATACACATCTCCGAGCCCACGAGACTCTGGATAATCTCGTATGCCGTC10.8130081300813009No Hit
TTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCGTCC10.8130081300813009No Hit
TTAACAGCCTGCCCACCCTGGAAACGGCTCAGCCGGAGGTAGGGTCCAGCG10.8130081300813009No Hit
GTGTATCTCTTTAGTTGCTTACTTTTCCTTGTATGAGACTGTATCTTATAC10.8130081300813009No Hit
GTATCAACGCAGAGTACGGGGTATCAACGCTGTCTCTTATACACATCTCCG10.8130081300813009No Hit
AACCCAGTGTGTTTCGACACACTATCATTAACTGAATCCATCGGTTAATGA10.8130081300813009No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTGAACCCCCTCCTT10.8130081300813009No Hit
AAACTCGGGTGTCCAGTGGCGCATCCCAAGTTCATCAAACGACCCTCAGCC10.8130081300813009No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACCTGTCTCTTATACAC10.8130081300813009No Hit
GAAATGGGAAGAGCGGCTTAAGCCATTAGGTGTAGGCGCTTTCCAAAGGTG10.8130081300813009No Hit
GCTTCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGTTGATACC10.8130081300813009No Hit
TTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCGTCT10.8130081300813009RNA PCR Primer, Index 40 (95% over 21bp)
CTTTGGTGGTATCGATGCTTTAGCACAGGAGTATGGACAAGGTATGGCCTG10.8130081300813009No Hit
TATCAACGCAGAGTACGGGCAGTGGTATCAACCCAAAAAAAAAAAAAAAAA10.8130081300813009No Hit
GATACCACTGCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCC10.8130081300813009No Hit
GTCTTAAAGAGAAGCTTCACGCTGCCTTCACTCTCTCCACCGGTCCTGATT10.8130081300813009No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAACCCCCCGTACCTT10.8130081300813009No Hit
GGGTAAAGCCAATGATTAGAGGCATTGGGGGCGCAACGCCCTCGACCTATT10.8130081300813009No Hit
CTTATACACATCTCCGAGCCCACGAGACTCTGGCCAATCTCGTATGCCGTC10.8130081300813009Illumina PCR Primer Index 4 (95% over 22bp)
TATCAAAGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAA10.8130081300813009No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTATAACCCCCGTTCC10.8130081300813009No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTACTCTGCGATAA10.8130081300813009No Hit
GCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGT10.8130081300813009No Hit
CATGCAAGTCGAACGGGATTTCATTTGTAGCAATACAAGTGGAGTTCAGTG10.8130081300813009No Hit
TTTCCGAATGGGGAAACCCAGTGTGTTTCGACTGTCTCTTATACACATCTC10.8130081300813009No Hit
GGTGTGCCCCCGGCGGCCCTTGAAAATCCGGAGGACCGAGTGCCGCTCACG10.8130081300813009No Hit
CCTACAGCCGACGCTAGGTCTCCATGCCAGCTGTCGCGCCACACCCGTACT10.8130081300813009No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTTT10.8130081300813009No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAAAAAAA10.8130081300813009No Hit
CCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGA10.8130081300813009No Hit
TCTGTACATTTTATCTGCCTTAACTGCATAGTTCCAATTCTAAGGAGAGTG10.8130081300813009No Hit
TTTCTCTTTAGTTTCCTCTTGCGGTGCAGACTCTTCTTTCTCAATCTTCTC10.8130081300813009No Hit
ATGTCGTTCATTAGTTTGCATTGTAGACGTTGTATCACCTGACAAACACCT10.8130081300813009No Hit
GGTATCAACGCAGAGTACGGGGCAGTGGTATCCGGTCTCTTATACACATCA10.8130081300813009No Hit
CACTGTACTTGAACCATCTCATGATGGTCGAGGAAAATCCCTGTCTCTTAA10.8130081300813009No Hit
TGGTAAGCCCTTGAAAGCGAAAGACGAAGTGACCCAAAACCCGACCAAAAA10.8130081300813009No Hit
GTACCCTACCTTGGACAAGGGTGATCGCGTTAAGGCGGAGCCGTCTCTTAT10.8130081300813009No Hit
TATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAA10.8130081300813009No Hit
ATAAGAGACAACAGCATAGCTGCCGAATACATGGTCCTCAACCTTCCAATA10.8130081300813009No Hit
GTTCGGGGGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCC10.8130081300813009No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10.8130081300813009No Hit
CTTATACACATCTCCGAGCCCACGAGACCCTGGGTAATCTCGTATGCCGTC10.8130081300813009No Hit
GCGCAGGGGGGAGATCACAGCTTCACCATCACGTTGCGGAGTTCCGTGTAG10.8130081300813009No Hit
ACATTACGCACGACATATTCTCATATGCTTTTCATGGGTGTTTAACCCTCT10.8130081300813009No Hit
AGGGTCTGTTGGGATTCAGGATTCTCATCCGGGCTTGGGTGTGATTCAAGC10.8130081300813009No Hit
CTTATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCGTC10.8130081300813009No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAACAAA10.8130081300813009No Hit
GGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCCTGTCTCTTATA10.8130081300813009No Hit
AAAAAGTACTCTGCGTTGATACCCGTACTCTGCGTTGATACCACCTGTCTC10.8130081300813009No Hit
GCTAAATACGGGCGAGAGACCGATAGCGAACAAGTACCGCGAGGTAAAGAT10.8130081300813009No Hit
GCATGTGCATTTTTGTGTACGGGGCTGTCACCCTGTATCGCACGCCTTTCC10.8130081300813009No Hit
TTATACACATCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCGTCT10.8130081300813009No Hit
CTTATACACATCTCCGAGCCCACGAGACCCTGGACAATCTCGTATGCCGTC10.8130081300813009No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTGTGTTTAAA10.8130081300813009No Hit
CGGTCCTCCTGGATGTGGGAAAACCCTTTTGGCCAAAGCTATTGCCAACGA10.8130081300813009No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGTGGCTGTCCCCTATAAA10.8130081300813009No Hit
GTAAAATCCCAGTCCTCGTCGACGGAGACTACAAAATCTTCGAGTCGCGTG10.8130081300813009No Hit
GTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG10.8130081300813009No Hit
GTATCAACGCAGAGTACGGGTGTGGTATCAACGCAAAGAAAAACTGTCTCT10.8130081300813009No Hit
GTCCTGAGAAGAGACCAACCGTTGACTTGGTCAAGGATAGAACTGTCTCTT10.8130081300813009No Hit
GAGTACGGGAAGAGTGGTATCAAACGCAGAAAAAAAAAAAAAAAAAAAAAA10.8130081300813009No Hit
TCACGGTACTTGTACGCTATCGGTCATTGAGGAATACTTAGGCTTAGAGGG10.8130081300813009No Hit
GGGCTACACACGTGCTACAATGGCGCATACAAAGAGAAGCGACCTCGCGAG10.8130081300813009No Hit
CGATAAATCTTTCCCCCAAAGGGCACATCCGGTATTAGCTCAAGTTTCCCT10.8130081300813009No Hit
GAAACGAATGGAATTATCATCGAATGGAATCGAATGGTATCTGTCTCTTAT10.8130081300813009No Hit
AGAAACGCCTGGTGCAGAGCCCCAATTCCTACTTCATGGATGTGCTGTCAC10.8130081300813009No Hit
GGTGTATCGCTCGACCTCGTCCCGGATCCATGCGGAGCATCGCCTGTCTCT10.8130081300813009No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTCTGTCTCTTATACACATCTCCG10.8130081300813009No Hit
TTACGATGAAATACATTGTTCAGTTTCTTTTGATTGTCTGTGTTTTGATCT10.8130081300813009No Hit
GTCAACATTGAGGATGAGATGAGGAGGAAGGCACAAGCTCAAAGAGAGGCT10.8130081300813009No Hit
GTACGGGAGAGTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGGCCTAA10.8130081300813009No Hit
CCTTGGAGGACCGAACCCACGTATGTGGCAAAATACGGCGACTGTCTCTTA10.8130081300813009No Hit
GTTTTTGTTTGGCACGGCTACTGCTGCGTTTCAGGTCGAAGGTTCAATTAA10.8130081300813009No Hit
GATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGA10.8130081300813009No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATAA10.8130081300813009No Hit
CATTTGTTTGCATTGCATGTTAGACTTCTCCAAATTAACAACTCTTTGGAT10.8130081300813009No Hit
GGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTCTGTCTC10.8130081300813009No Hit
CTTCCAATATACAACAAATACTGGCCCCTCATTGTTCTATTTTTTTACATC10.8130081300813009No Hit
GTATAGAACCAACCATTGTTCAAAATAGTACTAACCAAAAAACACCACAAA10.8130081300813009No Hit
CCTTGGTCCTTGCCTATATATGTGTGTAACACCTTATCTCTTACAATCTGT10.8130081300813009No Hit
AAAGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCAC10.8130081300813009No Hit
ACACTCCTCACGGTATGCCTTCGCCGCATATAGAACTCTCTCTTATACACA10.8130081300813009No Hit
GTATCAACGCAGAGTACGGGCAGTGGTATCAACGCAGAGTCTGTCTCTTAT10.8130081300813009No Hit
CTTATACACTTACACTTTATGCACAAAATGTAGGGTTATAATAATGTCTGT10.8130081300813009No Hit
CTCCAATCCGGACTACGACGCACTTTATGAGGTCCGCTTGCTCTCGCGAGG10.8130081300813009No Hit
GGGCAAAGGGAATGCCATCGGTGCTGGGACCTGTGAGCACTACAGGAGGAA10.8130081300813009No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCTTTGT10.8130081300813009No Hit
GCCCACCAAGGCGATGATCAGTAGCCGGTCTGAGAGGATGAACGGCCACAT10.8130081300813009No Hit
GCACTAGACAGCTTCCTGGCCTTGATAATGGCAGCACCTGTCTCTTATACA10.8130081300813009No Hit
ACCCAGAGTGCCTTTTTCTACAATAAACACATTGATTCCCTTCTGTCTCTT10.8130081300813009No Hit
TTATACACATCTCCGAGCCCACGAGACGACGCTATATCCCGTATGCCGTCT10.8130081300813009No Hit
CTTCCAGCCGCTGGACCCTACCTCCGGCTGAGCCGTTTCCAGGGCTGTCTC10.8130081300813009No Hit
CGTCTACTATTCTAGCATTCACTCCAATTCTTTCAGAAATTAATCTGTCCA10.8130081300813009No Hit
GAATATCACTGATAACCCTTCATATGTCATTCGTTGTTATCTGTTGATTTC10.8130081300813009No Hit
ACTGCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTG10.8130081300813009No Hit

[WARN]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers