FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0120_pd_92.351000000466cc.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0120_pd_92.351000000466cc.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences215185
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCGTC2425711.272625880056696No Hit
TCTTATACACATCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCGT53062.4657852545484116No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCG15400.7156632664916235No Hit
TTATACACATCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCGTCT8180.3801380207728234No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC27450.042.2937745
GCCGTCT1450.041.89525245
GACGTAG250.002103632335.99888220
CTTATGC250.002103632335.99888218
CTCTCGA250.002103632335.99888224
ATGCCGT33300.034.86378544
TATGCCG34600.033.5538743
GTATGCC34700.033.45717642
ATCTCGT34750.033.40903537
TCTCGTA34750.033.40903538
CACGAGA34850.033.3777321
CCACGAG34850.033.3777320
CTCGTAT34800.033.36103439
TCGTATG34800.033.36103440
CGAGACC34800.033.36103423
CCCACGA34900.033.32991419
GAGACCC34800.033.29638324
ACGAGAC34950.033.2822322
CGTATGC34900.033.26544641
AATCTCG34700.033.26265736
Produced by FastQC (version 0.11.2)