FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0120_pd_89.35100000046653.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0120_pd_89.35100000046653.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24912
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCGTC692727.80587668593449No Hit
TCTTATACACATCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCGT5972.3964354527938343No Hit
TTATACACATCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCGTCT2561.0276172125883107No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCG2110.8469813744380219No Hit
ATACACATCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCGTCTTC710.28500321130378936Illumina PCR Primer Index 7 (95% over 22bp)
TATACACATCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCGTCTT390.15655105973025046Illumina PCR Primer Index 7 (95% over 21bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC7550.040.2317945
TCCGAGC8000.037.9687513
CTCCGAG8000.037.9687512
GCCCACG8050.037.7329218
CCACGAG8050.037.7329220
TATGCCG8050.037.7329243
GAGCCCA8050.037.7329216
CACGAGA8050.037.7329221
CCCACGA8050.037.7329219
ACGAGAC8050.037.7329222
CTGGATA8050.037.7329230
CGTATGC8050.037.7329241
TCGTATG8050.037.7329240
TCTCCGA8050.037.7329211
CGAGACC8050.037.7329223
CTCGTAT8050.037.7329239
GAGACCC8050.037.7329224
AATCTCG8000.037.687536
ATCTCGT8000.037.687537
TCTCGTA8000.037.687538