FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0120_pd_88.35100000046639.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0120_pd_88.35100000046639.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences318021
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTC209326.581955279682789No Hit
TTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTCT33001.037667323855972RNA PCR Primer, Index 44 (95% over 22bp)
TCTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGT12360.38865357948060036No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAAC6270.19715679153263466No Hit
AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC5750.18080567006581325No Hit
ATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTCTTC5130.16131010216306468RNA PCR Primer, Index 44 (100% over 23bp)
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCC4210.1323811949525346No Hit
TATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTCTT4100.12892230387301468RNA PCR Primer, Index 44 (100% over 22bp)
CACAGCATGTGCATTTTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCCT3800.1194889645652331No Hit
GTATCAACGCAGAGTACGGGCAGCTGTCTCTTATACACATCTCCGAGCCCA3660.11508673955493505No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTC3540.11131340383182242No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCG3510.11037006990104427No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTTT3510.11037006990104427No Hit
CATGCTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATCCTGTCTGAAT3350.10533895560356078No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAG3230.10156561988044815No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC27000.037.1665445
GAGTTAT250.002103234436.0055351
ATGCCGT29100.034.48441744
CGTATGC29300.034.09544441
TATGCCG29550.033.95927443
AGACAAC29500.033.9405625
CGAGACA29500.033.9405623
GTATGCC29450.033.92178342
TCGTATG29400.033.90294340
ATCTCGT29500.033.8642937
TAATATC29550.033.80698833
GACAACA29550.033.80698826
CTCGTAT29500.033.78801739
AACATAA29400.033.7498829
TATCTCG29550.033.73084636
CATAATA29450.033.6925831
GAGACAA29900.033.63700524
ATATCTC29800.033.59887735
ACATAAT29600.033.5978630
CACGAGA29950.033.5808521
Produced by FastQC (version 0.11.2)