FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0120_pd_82.3510000004658a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0120_pd_82.3510000004658a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences272221
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTC6387323.46365636743675No Hit
TCTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGT271859.986371367381649No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCG55562.0409887554597184No Hit
TTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTCT15450.567553568607859RNA PCR Primer, Index 44 (95% over 22bp)
TCTTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCG2870.10542904478346637No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTTAA207.026194E-444.99687613
TGCCGTC68600.043.2586645
CGTCTTC301.1380991E-437.497445
GGGACAC250.002105007435.997515
GGATTAA250.002105007435.997510
TGCACTC250.002105007435.997512
GCCGTCT2700.035.8308545
ATGCCGT97600.030.3590644
CTATAAG300.005123730730.0199781
TATAATG300.00512833930.014462
GAACCAT300.00513295130.0089446
GTAGGGA300.00514218429.9979212
TAGGGAC300.00514218429.9979213
TGTAGGG300.00514218429.9979211
TATGCCG103750.028.4944143
CTCGTAT103550.028.46253639
ATCTCGT103400.028.46030837
TCTCGTA103300.028.44429838
CACGAGA104700.028.42925821
ACGAGAC104700.028.42925822
Produced by FastQC (version 0.11.2)