FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0120_pd_81.35100000046560.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0120_pd_81.35100000046560.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences123927
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTC2895923.36778910164855No Hit
TCTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGT50534.077400405077182No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCG13991.1288903951519846No Hit
TTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTCT6560.5293438879340256RNA PCR Primer, Index 44 (95% over 22bp)
ATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTCTTC3890.31389447013160976RNA PCR Primer, Index 44 (100% over 23bp)
TATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTCTT2070.16703381829625505RNA PCR Primer, Index 44 (100% over 22bp)
GAAAGAAAGAGAGAAAGAGAGAAAGAGAAAGGGAAAGGAAAAGAAAACAAA1400.11296973218104206No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTTC302.1498E-644.99999645
TGCCGTC32000.042.39843845
GGGAAAG403.4250297E-739.37531
ATGCCGT37250.036.4228244
GAGGAGT250.00209987636.06
TTAAGTG250.00209987636.04
TACAGGT250.00209987636.035
ATCTCGT38600.035.03238337
TATCTCG38450.034.993536
TCTCGTA38650.034.9870638
AGCCCAC38800.034.96778517
CCCACGA38800.034.96778519
GAGCCCA38800.034.96778516
TATGCCG38900.034.93573443
CGAGCCC38900.034.93573415
CGAGACA39050.034.9167723
CTCCGAG39250.034.91082812
CTCGTAT38800.034.90979439
ATATCTC38500.034.8896135
GCCCACG38900.034.8778918
Produced by FastQC (version 0.11.2)