H23LNBCXX_l01n01_bir_0120_pd_8.35100000045c37.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	H23LNBCXX_l01n01_bir_0120_pd_8.35100000045c37.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	18484
Sequences flagged as poor quality	0
Sequence length	51
%GC	44

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGTC	1493	8.07725600519368	RNA PCR Primer, Index 27 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGT	105	0.5680588617182428	No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA	69	0.37329582341484524	No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA	63	0.3408353170309457	No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAAC	62	0.33542523263362906	No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA	54	0.2921445574550963	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCG	53	0.2867344730577797	No Hit
TTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGTCT	50	0.2705042198658299	RNA PCR Primer, Index 38 (95% over 21bp)
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA	50	0.2705042198658299	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	48	0.2596840510711967	No Hit
CACAGCATGTGCATTTTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCCT	47	0.2542739666738801	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	45	0.2434537978792469	No Hit
GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	45	0.2434537978792469	No Hit
GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	45	0.2434537978792469	No Hit
CATATTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATCCTGTCTGAAT	42	0.22722354468729714	No Hit
CATGCTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATCCTGTCTGAAT	37	0.20017312270071413	No Hit
GGTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA	37	0.20017312270071413	No Hit
GTATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA	36	0.19476303830339753	No Hit
GAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	32	0.17312270071413113	No Hit
GCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATA	32	0.17312270071413113	No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	32	0.17312270071413113	No Hit
GTATCAACGCAGAGTACGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA	30	0.16230253191949795	No Hit
TATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA	29	0.15689244752218134	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTC	29	0.15689244752218134	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	28	0.15148236312486477	No Hit
GTACGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	25	0.13525210993291495	No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCA	24	0.12984202553559834	No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCACA	24	0.12984202553559834	No Hit
GGTATCAACGCAGAGTACGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAA	24	0.12984202553559834	No Hit
CCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCAC	24	0.12984202553559834	No Hit
GTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	23	0.12443194113828177	No Hit
GGTATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAA	22	0.11902185674096516	No Hit
TATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAA	22	0.11902185674096516	No Hit
CAGTATGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC	20	0.10820168794633195	No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAG	19	0.10279160354901536	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
TGCCGTC	155	43.548386	45
GCCCACG	180	38.750004	18
CACGAGA	180	38.750004	21
AGACGAA	180	38.750004	25
CGAGACG	180	38.750004	23
GAGACGA	180	38.750004	24
GCTATCT	175	38.571426	34
TATCTCG	175	38.571426	36
CGAATTG	175	38.571426	28
ATGCCGT	175	38.571426	44
GAATTGC	175	38.571426	29
TGCTATC	175	38.571426	33
ACGAATT	175	38.571426	27
CTATCTC	175	38.571426	35
ATTGCTA	170	38.38235	31
AATTGCT	170	38.38235	30
TTGCTAT	170	38.38235	32
AGCCCAC	185	37.702705	17
CCACGAG	185	37.702705	20
CCCACGA	185	37.702705	19

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content