FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0120_pd_77.351000000464db.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0120_pd_77.351000000464db.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26626
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTC277110.407120859310448No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGT6592.475024412228649No Hit
TTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTCT2991.1229625178397056Illumina PCR Primer Index 8 (95% over 21bp)
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAAC1590.5971606700217833No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACA780.29294674378427105No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAACGC760.28543528881544356No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCA710.2666566513933749No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTT640.24036655900247877No Hit
ATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTCTTC600.22534364906482388Illumina PCR Primer Index 8 (95% over 23bp)
TCTTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCG560.21032073912716892No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAG450.16900773679861789No Hit
GGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAA430.16149628182979042No Hit
TATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTCTT380.14271764440772178Illumina PCR Primer Index 8 (95% over 22bp)
AAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGT350.1314504619544806No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCG330.12393900698565313No Hit
AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC320.12018327950123939No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACCTGTCTCT300.11267182453241194No Hit
GGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCA300.11267182453241194No Hit
GAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAAC290.1089160970479982No Hit
CTTATATACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTC280.10516036956358446No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA280.10516036956358446No Hit
ATTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTC280.10516036956358446No Hit
GTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGG270.10140464207917073No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGGGG253.7886683E-545.03
TGCCGTC3500.039.85714345
GCCGTCT401.504258E-533.7545
ATGCCGT4300.032.4418644
AGCCCAC4550.032.14285717
GCCCACG4550.032.14285718
CCACGAG4550.032.14285720
CACGAGA4550.032.14285721
CCCACGA4550.032.14285719
AGACTTG4600.031.79347625
GACTTGC4600.031.79347626
GAGACTT4600.031.79347624
TTGCTGG4550.031.64835229
ACTTGCT4650.031.45161427
ACGAGAC4650.031.45161422
GCTGGAA4600.031.30434631
TGCTGGA4600.031.30434630
AATCTCG4250.031.23529436
ATCTCGT4250.031.23529437
CGTATGC4400.031.19318241
Produced by FastQC (version 0.11.2)