H23LNBCXX_l01n01_bir_0120_pd_76.351000000464ce.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	H23LNBCXX_l01n01_bir_0120_pd_76.351000000464ce.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	320495
Sequences flagged as poor quality	0
Sequence length	51
%GC	41

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTC	20954	6.538011513440148	No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGT	3021	0.9426044088051295	No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAAC	1857	0.5794162155415841	No Hit
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACC	929	0.2898641164448743	No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCA	893	0.2786314919109502	No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA	886	0.27644737047379836	No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAACGC	772	0.24087739278303874	No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA	735	0.22933275090095007	No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA	696	0.21716407432253232	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACA	684	0.21341986614455763	No Hit
CCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCAC	655	0.20437136304778547	No Hit
GCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGT	646	0.20156320691430443	No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAG	643	0.20062715486981078	No Hit
TATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA	595	0.185650322157912	No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTT	592	0.1847142701134183	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCG	557	0.17379366292765877	No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACCTGTCTCT	484	0.15101639651164603	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCC	463	0.14446403220019033	No Hit
TATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGC	442	0.13791166788873463	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTCAGT	435	0.13572754645158272	No Hit
GCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATA	409	0.1276150953993042	No Hit
GGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAA	368	0.11482238412455732	No Hit
GAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAAC	361	0.11263826268740541	No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA	356	0.11107817594658263	No Hit
ACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGG	340	0.1060858983759497	No Hit
TCATTAACCTATGGATTCAGTTAATGATAGCTGTCTCTTATACACATCTCC	326	0.10171765550164588	No Hit
AAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGT	323	0.10078160345715222	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGCCGTC	2180	0.0	42.41737	45
TTAAGGT	35	6.2232175E-6	38.58133	4
ATGCCGT	2505	0.0	36.91412	44
AGAGCTA	25	0.00210542	35.998	8
AATCTCG	2575	0.0	35.91063	36
ATCTCGT	2585	0.0	35.858746	37
TATGCCG	2585	0.0	35.858746	43
CACGAGA	2605	0.0	35.84254	21
CGTATGC	2590	0.0	35.78952	41
CCACGAG	2610	0.0	35.773876	20
GAATCTC	2585	0.0	35.77171	35
TCGTATG	2595	0.0	35.72056	40
GCTGGAA	2585	0.0	35.684673	31
CTCGTAT	2605	0.0	35.669804	39
TTGCTGG	2595	0.0	35.63386	29
TGCTGGA	2600	0.0	35.565334	30
CTGGAAT	2620	0.0	35.46559	32
GGAATCT	2615	0.0	35.447365	34
GAGACTT	2635	0.0	35.434467	24
GACTTGC	2625	0.0	35.398037	26

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content