FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0120_pd_73.35100000046461.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0120_pd_73.35100000046461.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences129195
Sequences flagged as poor quality0
Sequence length51
%GC36

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTC122619.490305352374317No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGT12030.9311505863230001No Hit
GATAGGCATCTATTTAGTTACTTATGTCAAATGAATAGCCAGTTGTTCTCA3800.2941290297612137No Hit
TTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTCT3500.2709083168853284Illumina PCR Primer Index 8 (95% over 21bp)
GTTGATACAGCAAAAATTATTGAAAAGACATTCCTTTTCCCTGAATTTCCT3230.2500096752970316No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCG3230.2500096752970316No Hit
ATGTTTAAGGCATCAGAATTTAGTTTAATTGCCTGAAGGAAGGAAAATTAA2680.2074383683579086No Hit
ATCATCAGGAAAGCATTTTGAAACATAGGTTATATCCATTGTCATCAATGT2680.2074383683579086No Hit
GATTTACAAAGAGTGTATTTCCAAACTGCTCGATTAAAGAAAGGTTAAACT2640.20434227330779056No Hit
GAGTGTTTCAAAACTACTCAATCAAAAGAAAAGGTCAACTCTTTGAGATGA2610.20202020202020202No Hit
ATTCTCTACAGCAGTGTATTAATAAGCACTATGCTTCAATGAATTGTGGTG2550.19737605944502495No Hit
ATGTTGGAAAAGGAAATATCTTCACATAAAAACCAGACAGAAGCATTCTCA2500.19350594063237742No Hit
ATTCCGGAATAGATACGGAAAAGATGATTGAGAAGAAACTAAAATTTGCTG2480.1919578931073184No Hit
ATTCCATTCCATTTGAAGATGATTCCATTCAAGACCATTCGATGATTGCAT2430.18808777429467086No Hit
ATAAACATTGGCATTATCTGTTTCAACTGACAGGAATTAATACTGAGTCAT2410.18653972676961184No Hit
TACTAGTTATGTCCTATTCTCTTCCTCATTTTGCTAGTGGAGAATACTAAT2110.16331901389372655No Hit
ATACTTACCAAGAAAATAGCAAAAGAAAGCAGGGATTGCAAATCCTAGTCT2060.15944889508107898No Hit
GCTAGAGTATTTAAGTTACTTGCTAATTCAGCAAAGAATGGATTCGATAGA2050.1586748713185495No Hit
GGTGACTACAGTCTAGAATACTGTATTGTTACTTGAAGTTTGCTAAGAGAA1980.15325670498084293No Hit
ATTAGTTTAACCCTTTTGAAGTTTGTTTTTGTTGAAGATGCAGAGAGACAC1950.15093463369325438No Hit
ATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTCTTC1920.14861256240566587Illumina PCR Primer Index 8 (95% over 23bp)
TTACAACATCTACATCAACATTGTCTTTGTCCCAATATTCATCGTTTTTCT1900.14706451488060684No Hit
GAATTATGATCAAATGGAATCGAATGTAATCATCATCAAATGGAATCAAAA1880.14551646735554782No Hit
GAAAATATTTGATTCAATCAAACAGCATTACTTGGTATGGTGTAGAGAAAA1810.14009830101784126No Hit
GTACAGTGTTCTGTAAAAATTTGGAATTTGTTGCTAACATTTTAAAACCAG1800.13932427725531174No Hit
TCTAACAACTGCACATGATTCTAACAAACTGATTATATGGCTTTGAAATGC1730.13390611091760518No Hit
TAGTTTCAGTTATGCAGGATGAATAAGCTTCTGAGGGAAAATATACATGGT1720.13313208715507566No Hit
ATAGTATTTTGTGGGACAAAGACTGGTAATTTCACAAATTGAATAAAGAAG1690.13081001586748714No Hit
ATATAATTCAACACAACACAACACAACACAACATGACTTAATGCACTACAC1640.1269398970548396No Hit
TCCTTTAAGATGCACAAAAACAATTTTCTTCTAGAACAACATACCTGCCAT1630.12616587329231008No Hit
GAATAGTCTCTTGGATGAATGGCTCTAGGAAAATTGTATATCCACATGCAA1610.12461782576725106No Hit
GTATAGTGTTTCAGTAGTCCTGATACATTTTACTGAAGATTTGATCTTGGT1600.12384380200472156No Hit
ATCTATGGTGATATCTACTCTATCATTCCTGATGTTGCTAGTTTGTTTTTC1590.12306977824219205No Hit
TTGTAAATATTGAGCAAATTGCTCAATTTTTTTATCGATGATTTTCACTTG1580.12229575447966254No Hit
TATTTATGCAGTAGGTTTTAATATCGCCATAAGTGTCTTCATTACAAGCAA1570.12152173071713303No Hit
ATATACAAGTTGCAGGAAAACATCTCTATTACACCCTAATTACTACATATC1560.12074770695460352No Hit
TATTTAAATGGATTCAGTGCTTACCATCATATCTTTTCCATGGCTTTTCTA1550.11997368319207401No Hit
ATATATTGGTGTGTGATTTTCTTTTCTTGTGTGATGTATTTGTCTGGCTTT1540.1191996594295445No Hit
ATATAAACCTTGTTGTACCATCTTCACTGCTTTTTTAAAGTTACCATTCCA1520.11765161190448548No Hit
GGTAGGAAATGAACATATGTTTTGAAGCTTTTGTAACTGATCTTCTACTCA1500.11610356437942645No Hit
ATTCTTTTGTGGCTAAATTCAATGAATCTTGTGAAAATTTGTTGACTGAAA1490.11532954061689694No Hit
ATCAAAAGAATCCAGCTAACCTAACTTGAAAAAAATCCCAATTCAAAAATT1490.11532954061689694No Hit
GATAAAAAGGGATACCCATGTCTCTATTAACAGCTTTATCTCTTTCTACAG1480.11455551685436743No Hit
TTTAACTAAGTACTGAAATCCTGCAAAAAAGAATAGCTTTTGGTATTTTCC1460.11300746932930841No Hit
TCATAAAACACACATATTTTGTAGAGTGAGAAGCAAGAAAGATTTGATTCA1440.1114594218042494No Hit
AATAGGTGTCTATTTACAAACTCTCTTGTGTCTCTCAGAGTAGTTATTTTC1430.1106853980417199No Hit
GCTATTGAGGGTATAAAGATCTTTGAGAAGCTTATACAGCTATGTCTTGTG1410.10913735051666087No Hit
TCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCT1410.10913735051666087No Hit
GTACAAAGTTTTAGAGACAGGCCAGATATATCTGCATTAGCCTTGAGAAAA1380.10681527922907234No Hit
AGACTATTCACACTGATGAATACTTTATTGAGTACACATTTCAACTTTGAC1380.10681527922907234No Hit
TGAATAAACAAAGAACATGGATTCTTCAGATGGGATCTACTCTTGGTGAAG1380.10681527922907234No Hit
TTTTTGTTTTTGGTTTATTTTTGTTGTTGTTGTTGTTGCATGAAATCTTAA1370.10604125546654283No Hit
AGCATTAGTCACAATAGTCAAAAAGGGGAAACAACCTAAATGTTCATCAAC1360.10526723170401332No Hit
GGAATGGAATGGAATGGAATAGAATGGAATGGAATGGAATCGAATGGAATG1350.10449320794148381No Hit
TGTTTATGCATTGATTTTGTACCCTGAAACTTTGCTGAAGTCATTTATCAG1330.10294516041642479No Hit
CAGAATAGTTAAGGAAATGGTGCTTGAGAATTTTCTAAAGTTAACTCCAAA1310.10139711289136577No Hit
ATTGGTAGGAGATTTCATTGTCCTTCTTATTTTTCCTTCAAGGTTATAAAG1300.10062308912883626No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTAG206.998435E-445.0151061
CCGGAAT206.998435E-445.0151064
TGGTAGG206.998435E-445.0151063
AACGTTG207.011808E-444.9976834
TGTTTCG207.011808E-444.9976821
ACTATCG207.011808E-444.9976843
TTGGAGT207.011808E-444.9976814
GTTTCGC207.011808E-444.9976822
TTAGCCC207.011808E-444.9976810
AGCTTAG207.011808E-444.9976834
TGTGGAG207.011808E-444.9976841
TAGCGCT207.011808E-444.9976820
ATAGCGC207.011808E-444.9976819
ATTAACG207.011808E-444.9976831
CACTGCT207.011808E-444.9976825
TCGATAG207.011808E-444.9976844
TGTGAGG207.011808E-444.9976810
CAGGGAG207.011808E-444.9976810
ACCTACC207.011808E-444.997688
CATAGGT207.011808E-444.9976824
Produced by FastQC (version 0.11.2)