FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0120_pd_72.35100000046448.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0120_pd_72.35100000046448.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences150629
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTC72754.829747259823805No Hit
TTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTCT11450.7601457886595543Illumina PCR Primer Index 11 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGT7060.4687012461079872No Hit
ATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTCTTC3190.21177860836890638Illumina PCR Primer Index 11 (95% over 23bp)
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACCTGTCTCT2910.19318989039295223No Hit
CATGCTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATCCTGTCTGAAT2030.13476820532566772No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGCTGTCTCTTATACACATCT1880.12480996355283512No Hit
GGGCTAAACCATGCACCGAAGCTGCGGCAGCGACGCTTATGCGTTGTTGGG1750.11617948734971353No Hit
TATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTCTT1740.11551560456485803Illumina PCR Primer Index 11 (95% over 22bp)
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTC1720.11418783899514702No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTTT1710.11352395621029152No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTT1670.11086842507086948No Hit
CACAGCATGTGCATTTTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCCT1590.10555736279202543No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGTAT301.13521775E-437.50000429
CAATGCG250.002101312436.026
ATAGTTA250.002101312436.06
TGCCGTC10550.035.40284345
CGAACCG401.5516136E-533.75000431
ATGCCGT11450.032.6200944
ATCTCGT11350.032.51101337
CGCTATA11500.032.4782631
AGACGAC11650.032.4463525
GGTATCA3750.032.41
CGTATGC11600.032.3922441
CACGAGA11600.032.3922421
GAGACGA11600.032.3922424
TATCTCG11400.032.3684236
TATGCCG11550.032.3376643
ACGACGC11700.032.30769327
TCGTATG11650.032.2532240
CGAGACG11650.032.2532223
GACGCTA11650.032.2532229
GGGGAAG352.8088293E-432.14285718
Produced by FastQC (version 0.11.2)