FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0120_pd_71.3510000004642e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0120_pd_71.3510000004642e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences511215
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTC56321.101689113191123No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCTT6620.12949541777921228No Hit
TCTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGT6190.1210840839959704No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC6750.040.6637545
TCGTATG7350.037.03816240
CGTATGC7450.036.84341
CGAGACG7550.036.65658623
CGCTATA7500.036.60095231
TATGCCG7500.036.59737443
GACGCTA7600.036.4154229
ACGCTAT7550.036.35856230
ATGCCGT7550.036.3550144
CTCGTAT7550.036.0570239
GGCGTAT250.002106757835.9974214
AGACGAC7700.035.6502825
ATCTCGT7700.035.64679337
ACGACGC7750.035.4202827
TATCTCG7700.035.35460736
GAGACGA7850.035.25569524
TCTCGTA7800.035.18978538
GACGACG7850.034.96906726
CACGAGA7900.034.7477421
CCCACGA8000.034.3100419
Produced by FastQC (version 0.11.2)