FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0120_pd_70.35100000046404.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0120_pd_70.35100000046404.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences151871
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTC123018.099637192090656No Hit
TCTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGT55183.6333467218889712No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACA18881.243160313687274No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTT10770.7091544797887681No Hit
AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC4520.29762100730224994No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAA4260.2805012148468108No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCG4220.27786740062289705No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACCTGTCTCT3900.25679688683158736No Hit
TTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTCT3710.24428626926799715Illumina PCR Primer Index 11 (95% over 21bp)
AAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCCA3590.236384826596256No Hit
GTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCAC3190.21004668435711887No Hit
CATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTTT3110.2047790559092914No Hit
GGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCA3010.19819452034950716No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACCTGTCTCTTATACAC2680.176465553002219No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAAC2540.16724720321852096No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACCTGTCTCTTATAC2300.15144431787503868No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTC2250.14815205009514654No Hit
CACTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGA2110.13893370031144853No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCC1950.12839844341579368No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA1840.12115545430003095No Hit
CATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACCTGTCTCTT1790.1178631865201388No Hit
CATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATC1710.11259555807231138No Hit
AAAAAGTACTCTGCGTTGATACCACCTGTCTCTTATACACATCTCCGAGCC1600.10535256895654865No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC13900.043.53743445
TAAGGTC250.002102725735.9952634
GCCGTCT458.642892E-734.99538845
ATGCCGT19050.031.64937444
CGTATGC19350.031.27494841
TCGTATG19250.031.20368240
TATGCCG19400.031.19434543
CGCTATA19250.031.09705531
ACGCTAT19350.031.05264730
CTCGTAT19200.031.05059839
CACGAGA19450.031.00869821
CGAGACG19450.031.00869823
GACGACG19450.031.00869826
ACGAGAC19450.031.00869822
ACGACGC19450.031.00869827
ATCTCGT19100.030.97759437
GACGCTA19400.030.97261429
AGACGAC19500.030.92918825
CCACGAG19500.030.92918820
GAGACGA19600.030.88620624
Produced by FastQC (version 0.11.2)