H23LNBCXX_l01n01_bir_0120_pd_66.3510000004638c.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	H23LNBCXX_l01n01_bir_0120_pd_66.3510000004638c.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	228793
Sequences flagged as poor quality	0
Sequence length	51
%GC	42

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTC	12702	5.551743278859056	No Hit
TCTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGT	5068	2.2151027347864662	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACA	940	0.4108517306036461	No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTT	921	0.40254728072974266	No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACCTGTCTCT	817	0.35709134457784986	No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAAC	635	0.27754345631203753	No Hit
AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC	599	0.2618087091825362	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCG	548	0.23951781741574263	No Hit
GTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCAC	479	0.20935955208419837	No Hit
CATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACCTGTCTCTT	414	0.18094959198926538	No Hit
AAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCCA	356	0.15559916605840213	No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGCTGTCTCTTATACACATCT	331	0.14467225832958175	No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTC	314	0.1372419610739839	No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACCTGTCT	302	0.13199704536415013	No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAA	290	0.12675212965431634	No Hit
GGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCA	282	0.12325551918109381	No Hit
AACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACCTGTCTCTTATAC	276	0.12063306132617693	No Hit
CACTATCATTAACTGAATCCATAGGTTAATGACTGTCTCTTATACACATCT	273	0.11932183239871849	No Hit
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACC	266	0.11626229823464879	No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCA	263	0.11495106930719033	No Hit
TCATTAACCTATGGATTCAGTTAATGATAGCTGTCTCTTATACACATCTCC	255	0.11145445883396783	No Hit
CTATCATTAACTGAATCCATAGGTTAATGACTGTCTCTTATACACATCTCC	246	0.10752077205159248	No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACCTGTCTCTTATACAC	243	0.10620954312413405	No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAACGC	235	0.10271293265091153	No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGACTGTCTCTTATAC	233	0.10183878003260588	No Hit
AAAAAAAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGA	230	0.10052755110514745	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGCCGTC	1245	0.0	43.734726	45
GCCGTCT	40	1.5551805E-5	33.74984	45
TGTCGAC	35	2.8134455E-4	32.1427	33
ATGCCGT	1745	0.0	31.203287	44
TACTAGA	30	0.0051388657	29.999855	2
TATCTCG	1845	0.0	29.634003	36
ATCTCGT	1850	0.0	29.55391	37
TATGCCG	1850	0.0	29.55391	43
CGCTATA	1855	0.0	29.474253	31
CGTATGC	1860	0.0	29.39502	41
TCGTATG	1855	0.0	29.352959	40
ACGCTAT	1865	0.0	29.316212	30
CTATATC	1885	0.0	29.243887	33
TCTCGTA	1870	0.0	29.237825	38
GCTATAT	1880	0.0	29.201988	32
CGAGACG	1880	0.0	29.201988	23
ATATCTC	1875	0.0	29.159857	35
GTATGCC	1875	0.0	29.159857	42
GACGCTA	1875	0.0	29.159857	29
ACGACGC	1875	0.0	29.159857	27

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content