FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0120_pd_65.35100000046362.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0120_pd_65.35100000046362.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences232161
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTC146826.3240595965730675No Hit
TCTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGT32951.4192736936867087No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCG5960.2567183980082787No Hit
TTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTCT4120.1774630536567296Illumina PCR Primer Index 11 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCATA207.0209795E-445.00086234
CGCATAG207.0209795E-445.00086235
GGAAGCG207.0209795E-445.00086229
CGATCGA207.0209795E-445.00086226
AGGAAGC253.885661E-544.9911710
GGAGTGG207.0284365E-444.991178
GCTCGCT207.0284365E-444.9911740
TACCGTG207.0284365E-444.9911744
TCAGTCG207.0284365E-444.9911710
TGCCGTC16750.042.9766445
ACGGATC250.002099009736.016216
ATACGGA250.002099009736.016212
TAACGCA250.002103455636.00068732
AGCTTCG250.002105681235.99293514
CGGATCA250.002105681235.9929357
CTCGCTA250.002105681235.99293541
CAGCTCG250.002105681235.99293538
AGCTCGC250.002105681235.99293539
ATGCCGT20250.035.5485844
CTCGTAT20600.034.944639
Produced by FastQC (version 0.11.2)