FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0120_pd_62.351000000462f7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0120_pd_62.351000000462f7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences321456
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCGTC90432.813137723358718No Hit
TCTTATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCGT16950.5272883380618187No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACCTGTCTCT15240.4740928774077945No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTT9140.2843312926185854No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACA7290.2267806480513663No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGCTGTCTCTTATACACATCT6890.2143372654422378No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACCTGTCT6830.21247075805086854No Hit
CTATCATTAACTGAATCCATAGGTTAATGACTGTCTCTTATACACATCTCC5840.1816733860932756No Hit
TCATTAACCTATGGATTCAGTTAATGATAGCTGTCTCTTATACACATCTCC5740.17856254044099348No Hit
CACTATCATTAACTGAATCCATAGGTTAATGACTGTCTCTTATACACATCT5600.17420735652779853No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAAC5190.16145288935344185No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA5010.15585336717933404No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCC4490.13967696978746702No Hit
CATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACCTGTCTCTT4490.13967696978746702No Hit
AACCCAGTGTGTTTCGACACACTATCATTAACTGAATCCATAGGTTAATGA4300.133766363048131No Hit
GTGTTTCGACACACTATCATTAACTGAATCCATAGGTTAATGACTGTCTCT4140.12878901000447962No Hit
TCGACACACTATCATTAACTGAATCCATAGGTTAATGACTGTCTCTTATAC4110.12785575630879498No Hit
CATGCTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATCCTGTCTGAAT3800.11821213478672042No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACCTGTCTCTTA3740.11634562739535116No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA3620.11261261261261261No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCACA3540.11012393609078693No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTTT3470.10794634413418944No Hit
ACCCAGTGTGTTTCGACACACTATCATTAACTGAATCCATAGGTTAATGAC3270.10172465282962521No Hit
GTGTTTCGACACACTATCATTAACTGAATCCATAGGTTAATGACTGTCCCC3260.101413568264397No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC10350.043.25638245
ATGCCGT12250.036.54723444
GGCGAAT12300.036.22702832
CGTATGC12400.036.10512541
TATGCCG12450.035.9601343
GCGAATC12400.035.9348733
ATCTCGT12450.035.77942337
CGAGACC12600.035.71613723
TCGTATG12550.035.6735940
CGAATCT12450.035.60425634
CCACGAG12700.035.42939420
CACGAGA12750.035.29594821
CTCGTAT12700.035.25224739
CAAGGCG12750.034.94842529
AATCTCG12800.034.8010836
GAGACCA13000.034.6171824
CCCACGA13000.034.61179719
GTATGCC12950.034.57170542
ACGAGAC13100.034.35292422
GACCAAG13050.034.31746326
Produced by FastQC (version 0.11.2)