FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0120_pd_61.351000000462dd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0120_pd_61.351000000462dd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63319
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCGTC24413.8550829924667163No Hit
TCTTATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCGT4740.7485904704748969No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAAC1920.3032265196860342No Hit
TTATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCGTCT1650.2605852903551857No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACCTGTCTCT1360.21478545144427422No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTT1210.1910958795938028No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAACGC1030.1626683933732371No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACA1030.1626683933732371No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCA930.14687534547292283No Hit
CATGCTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATCCTGTCTGAAT720.11370994488226283No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC2850.039.4729945
CCCACGA3550.031.68958719
CCACGAG3550.031.68958720
CGAGACC3550.031.05579423
GCCCACG3650.030.82137518
CACGAGA3650.030.82137521
CGAGCCC3800.030.78893515
ATCTCGT3600.030.62446437
TAACCTA1250.030.5994635
CGTATGC3700.030.40487141
TATGCCG3700.030.40487143
GTATGCC3700.030.40487142
ATGCCGT3700.030.40487144
ACGAGAC3700.030.40487122
GAGACCA3750.029.99947424
CCGAGCC3900.029.99947414
AATCTCG3600.029.99947436
CGAATCT3600.029.99947434
GGCGAAT3450.029.99947432
AGCCCAC3850.029.80467217