FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0120_pd_6.35100000045c00.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0120_pd_6.35100000045c00.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences626190
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAAC338615.407464188185694No Hit
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACC169472.7063670770852295No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCA156402.497644484900749No Hit
CCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCAC136332.177134735463677No Hit
GCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGT104271.66514955524681No Hit
GCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATA96331.5383509797345853No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAG80361.2833165652597454No Hit
CTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATAC63871.0199779619604272No Hit
CTTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGTC61890.9883581660518374RNA PCR Primer, Index 27 (95% over 21bp)
ACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGG53930.8612401986617481No Hit
TATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGC51870.8283428352416997No Hit
AAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGT46450.7417876363404079No Hit
CAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACG45580.7278940896533No Hit
GGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAA44670.7133617592104633No Hit
CCACTGCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGT42230.6743959501109887No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAACGC41050.6555518293169805No Hit
GTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGG39020.623133553713729No Hit
ACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCACTGCTT37320.5959852440952427No Hit
ACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGC34520.5512703811942062No Hit
GCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTAC34510.551110685255274No Hit
CTGCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGA29690.4741372426899184No Hit
ATACCACTGCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTG28030.44762771682716107No Hit
GTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTAC25600.4088216036666188No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAG24510.391414746323001No Hit
TACCACTGCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGC23650.37768089557482554No Hit
ATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCA20240.32322458039892044No Hit
GATACCACTGCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCT18950.30262380427665725No Hit
GAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAAC18470.2949583992079082No Hit
CTTCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGTTGATACCA18300.2922435682460595No Hit
GGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGA18300.2922435682460595No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCC15980.25519411041377216No Hit
GCTTCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGTTGATACC15730.2512017119404653No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACG15260.24369600281064852No Hit
GCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTA14410.2301218480014053No Hit
GTATCAAGCAGTGGTATCAAGCAGTGGTATCAAGCAGTGGTATCAAGCAGT14140.22581005765023396No Hit
TCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGTTGATACCACT13700.21878343633721392No Hit
GCTTGATACCACTGCTTGATACCACTGCTTGATACCACTGCTTGATACCAC13500.2155895175585685No Hit
TGATACCACTGCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTC12850.20520928152797077No Hit
CTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCACTGCTTCCC12500.19961992366534118No Hit
GTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCACTGC12380.1977035723981539No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA12080.19291269423018573No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAACTGTCTCTTATACA11250.17965793129880708No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTC10770.17199252623005798No Hit
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACCTGTCTCTTATACA10660.17023587090180295No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAACG9830.1569811079704243No Hit
CGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCA9590.15314840543604977No Hit
GCGTTGATACCACTGCTTCCGTACTCTGCGTTGATACCACTGCTTCCGTAC9380.14979479071847202No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTTT8940.142768169405452No Hit
GTACGGGGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAGTCAGA8740.13957425062680656No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCACA8510.13590124403136428No Hit
CTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCACTGCTTC8360.13350580494738018No Hit
CACAGCATGTGCATTTTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCCT7530.12025104201600152No Hit
TCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAG7510.11993165013813697No Hit
GTATCAACGCAGAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCA7290.11641833948162698No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA7090.11322442070298153No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA7090.11322442070298153No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATAA6970.11130806943579424No Hit
CTGCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATAC6950.1109886775579297No Hit
CATGCTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATCCTGTCTGAAT6890.11003050192433605No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGATG6870.10971111004647151No Hit
CTTCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACC6720.10731567096248742No Hit
CATATTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATCCTGTCTGAAT6580.1050799278174356No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGTG6490.10364266436704514No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAA6470.10332327248918059No Hit
GTGGTATCAAGCAGTGGTATCAAGCAGTGGTATCAAGCAGTGGTATCAAGC6320.10092783340519651No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC7300.037.59953745
TCGTATG7550.036.95049340
CTCGTAT7500.036.89685439
ATCTCGT7450.036.842537
TATGCCG7450.036.842543
CGTATGC7500.036.5968841
GTATGCC7500.036.5968842
ATGCCGT7500.036.5968844
ATTGCTA7500.036.30270431
TATCTCG7500.036.29690636
CGAATTG7550.036.0622928
ACGAATT7550.036.0622927
GCTATCT7500.036.0026834
AGACGAA7700.035.94423725
TTGCTAT7400.035.8810532
GACGAAT7600.035.8250426
GAGACGA7750.035.71233724
CGAGACG7750.035.71233723
TGCTATC7500.035.7026633
CTATCTC7650.035.5908935
Produced by FastQC (version 0.11.2)