FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0120_pd_57.35100000046257.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0120_pd_57.35100000046257.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22502
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCGTC269411.972269131632745No Hit
TCTTATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCGT2411.0710159096969158No Hit
TTATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCGTCT2220.9865789707581549No Hit
ATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCGTCTTC1390.6177228690783042RNA PCR Primer, Index 46 (95% over 22bp)
TATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCGTCTT690.3066394098302373RNA PCR Primer, Index 46 (95% over 21bp)
CTCTTATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCG640.2844191627410897No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC3350.034.92537345
GAGCCCA3700.033.44594616
CGAGCCC3700.033.44594615
AATCTCG3600.033.12536
ATCTCGT3600.033.12537
GCCCACG3600.033.12518
CCCACGA3600.033.12519
TCTCGTA3600.033.12538
AGCCCAC3750.033.017
CCGAGCC3750.033.014
TCTCCGA3750.033.011
TCCGAGC3750.033.013
CTCCGAG3750.033.012
CCACGAG3550.032.95774520
GGCGAAT3550.032.95774532
CACGAGA3550.032.95774521
ACGAGAC3550.032.95774522
GAGACCA3550.032.95774524
GCGAATC3650.032.67123433
ATCTCCG3800.032.56579210
Produced by FastQC (version 0.11.2)