FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0120_pd_56.3510000004623d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0120_pd_56.3510000004623d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences246773
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTC174147.056687725156317No Hit
TCTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGT34401.393993670296183No Hit
TTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTCT13530.548277161601958No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACCTGTCTCT10920.4425119441754163No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTT6960.28204057980411146No Hit
AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC4760.19288982182005324No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAAC4420.17911197740433515No Hit
TCATTAACCTATGGATTCAGTTAATGATAGCTGTCTCTTATACACATCTCC4130.16736028657916385No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACCTGTCT4030.1633079793980703No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCG3890.1576347493445393No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACA3810.15439290359966448No Hit
CATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACCTGTCTCTT3670.1487196735461335No Hit
CTATCATTAACTGAATCCATAGGTTAATGACTGTCTCTTATACACATCTCC3230.13088952194932185No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGCTGTCTCTTATACACATCT3120.12643198405011893No Hit
CACTATCATTAACTGAATCCATAGGTTAATGACTGTCTCTTATACACATCT3040.1231901383052441No Hit
ATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTCTTC2940.11913783112415054Illumina PCR Primer Index 4 (95% over 22bp)
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTC2890.11711167753360376No Hit
GTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCAC2620.10617044814465115No Hit
GTACGGGGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAGTCAGA2600.10535998670843244No Hit
CATGCTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATCCTGTCTGAAT2590.10495475599032308No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACCTGTCTCTTA2530.10252337168166696No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCC2520.1021181409635576No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGACTGTCTCTTATAC2510.10171291024544825No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC21000.041.14174345
ATGCCGT23850.036.131144
AATCCAC250.002104188535.9990312
TATGCCG24150.035.77543343
CGTATGC24050.035.7370841
GCCGTCT1700.035.7343345
CTCGTAT24100.035.4762239
TCGTATG24100.035.4762240
TATCTCG24050.035.45642536
ACCTATC24250.035.4423433
AGACGAT24000.035.43654625
GACGATA23900.035.39653426
CGAGACG24250.035.3495623
ATCTCGT24200.035.32962437
CGATAAC23950.035.32263628
GAGACGA24300.035.2768324
CACGAGA24400.035.22445721
CCACGAG24400.035.22445720
CCCACGA24500.035.1725219
GTATGCC24500.035.1725242
Produced by FastQC (version 0.11.2)