H23LNBCXX_l01n01_bir_0120_pd_52.351000000461c1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	H23LNBCXX_l01n01_bir_0120_pd_52.351000000461c1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	473876
Sequences flagged as poor quality	0
Sequence length	51
%GC	43

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTC	21815	4.603524972777689	No Hit
TCTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGT	3135	0.6615654728241143	No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTT	1197	0.2525977259873891	No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACCTGTCTCT	1151	0.24289054520591885	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACA	1105	0.2331833644244486	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCC	1038	0.219044644590568	No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAAC	999	0.21081464349323453	No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA	740	0.15615899518017373	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTCAGT	693	0.14624078872954105	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCG	681	0.1437084806995923	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTTT	646	0.1363225822789084	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTC	595	0.12556027315162616	No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGCTGTCTCTTATACACATCT	590	0.12450514480581419	No Hit
TCATTAACCTATGGATTCAGTTAATGATAGCTGTCTCTTATACACATCTCC	576	0.12155078543754061	No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCACA	552	0.1164861693776431	No Hit
GTACGGGGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAGTCAGA	532	0.11226565599439516	No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA	494	0.10424668056622409	No Hit
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACC	493	0.1040356548970617	No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCA	489	0.10319155222041208	No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA	479	0.10108129552878811	No Hit
CATGCTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATCCTGTCTGAAT	475	0.10023719285213854	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGATG	475	0.10023719285213854	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGCCGTC	2290	0.0	43.628036	45
ACCGCAA	30	1.1397744E-4	37.495163	10
ATGCCGT	2680	0.0	37.27918	44
GTCTGCG	25	0.002106104	35.999153	21
TATGCCG	2775	0.0	35.999153	43
TATTGCG	25	0.002106104	35.999153	19
CGTATGC	2780	0.0	35.853477	41
CTCGTAT	2775	0.0	35.833214	39
GACGATA	2790	0.0	35.805614	26
TATCTCG	2765	0.0	35.800083	36
CGAGACG	2810	0.0	35.710907	23
CGATAAC	2785	0.0	35.70832	28
ATCTCGT	2785	0.0	35.70455	37
TCGTATG	2800	0.0	35.59738	40
AGACGAT	2815	0.0	35.567547	25
ACGATAA	2810	0.0	35.55077	27
GATAACC	2800	0.0	35.436672	29
CCTATCT	2790	0.0	35.402397	34
GAGACGA	2830	0.0	35.37903	24
CACGAGA	2845	0.0	35.350666	21

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content