FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0120_pd_51.3510000004619b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0120_pd_51.3510000004619b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences167743
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTC6642139.59688332747119No Hit
TCTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGT120287.170492956487006No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCG101026.022307935353488No Hit
TTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTCT3420.2038833215096904No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC69950.044.41924745
ATGCCGT82050.037.86869444
ATCTCGT90850.033.9529737
CTCGTAT90850.033.9529739
TCTCGTA90850.033.92820438
TCGTATG91150.033.86590640
TATCTCG91050.033.8536836
CGTATGC91450.033.85321841
TATGCCG91800.033.84669543
GTATGCC91750.033.81609342
CTATCTC91400.033.77327335
CCTATCT91750.033.76704834
ACCTATC92200.033.74865733
AACCTAT92400.033.67560632
TAACCTA92550.033.6696531
CCCACGA93050.033.58544519
GCCCACG93100.033.5674118
GACGATA93150.033.54939326
CGAGACG93300.033.54368223
ACGAGAC93300.033.54368222