FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0120_pd_50.35100000046171.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0120_pd_50.35100000046171.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences375355
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTC348329.279748504748838No Hit
TCTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGT172604.598313596462016No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACA17430.4643604054828096No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTT16350.4355876436972999No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCG16230.43239067016557664No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACCTGTCTCT9830.2618854151403338No Hit
AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC9480.25256090900614087No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAAC7600.2024749903424758No Hit
GTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCAC7320.19501538543512142No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAA5540.1475936113812258No Hit
GGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCA5380.14333098000559472No Hit
AAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCCA5240.1396011775519175No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTC4580.12201782312743936No Hit
TTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTCT3920.1044344687029612No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACCTGTCTCTTATACAC3880.10336881085905342No Hit
CATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTTT3780.10070466624928401No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCCCC253.8870607E-544.99746745
TGCCGTC34850.043.5126245
CGTCTTG250.002105886635.99797434
TAACCGA250.002105886635.99797423
TCTAACG250.002105886635.99797428
GCCGTCT1050.034.28378745
CGGGGGG352.81111E-432.1538964
GGACCGT352.81111E-432.1538966
GACAATA352.8177124E-432.1410510
TTAACCG352.8177124E-432.1410522
ACCGAAT300.005144310629.99831225
CCGAATA300.005144310629.99831226
TGTCGAC453.485987E-529.99831233
GACTAGA300.005144310629.99831228
ATGCCGT53950.028.14948344
TATGCCG55600.027.27364543
ATCTCGT55450.027.18512337
TATCTCG55450.027.18512336
TCGTATG55700.027.18428840
CGTATGC55800.027.17589241