FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0120_pd_49.35100000046158.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0120_pd_49.35100000046158.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences205313
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTC6413331.236697140463587No Hit
TCTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGT2803413.654274205724917No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCG38821.8907716510888253No Hit
TTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTCT12520.609800645843176No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCCATA207.0197415E-445.09
TGCCGTC68900.043.5304845
GCCGTCT2150.041.86046645
GACTTTT352.8123657E-432.142867
TTATACT352.8123657E-432.142864
ATGCCGT99000.030.31818244
TCCATAC300.00513752930.00000210
TATCTCG102050.029.19157236
TATGCCG102950.029.17678643
CTCGTAT102600.029.14473739
GCCCACG103200.029.12790918
CTATCTC102200.029.12671335
CGAGCCC103100.029.1125115
CCTATCT102350.029.1060134
TCGTATG102600.029.10087840
ATCTCGT102450.029.09956237
CGTATGC103000.029.0970941
AGCCCAC103250.029.09200917
CCGAGCC103250.029.09200914
AACCTAT102800.029.08803632
Produced by FastQC (version 0.11.2)