FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0120_pd_48.3510000004613e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0120_pd_48.3510000004613e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences203801
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGTC128676.313511709952356RNA PCR Primer, Index 46 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGT22481.1030367858842693No Hit
TTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGTCT7800.3827262869171398RNA PCR Primer, Index 18 (95% over 21bp)
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACCTGTCTCT7090.3478883813131437No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAAC5880.28851673936830535No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTT3790.1859657214635846No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCG3150.15456253894730643No Hit
AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC3030.1486744422255043No Hit
CACTATCATTAACTGAATCCATAGGTTAATGACTGTCTCTTATACACATCT3000.14720241804505374No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA2960.14523971913778636No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGCTGTCTCTTATACACATCT2860.14033297186961793No Hit
TCATTAACCTATGGATTCAGTTAATGATAGCTGTCTCTTATACACATCTCC2840.13935162241598423No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCA2620.1285567784260136No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACCTGTCT2520.12365003115784516No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTTT2490.12217800697739462No Hit
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACC2480.12168733225057778No Hit
GTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCAC2430.11923395861649354No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAG2400.117761934436043No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAACGC2330.11432721134832509No Hit
CTATCATTAACTGAATCCATAGGTTAATGACTGTCTCTTATACACATCTCC2270.111383162987424No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACA2240.10991113880697347No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA2130.10451371681198818No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA2110.10353236735835447No Hit
CATGCTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATCCTGTCTGAAT2090.10255101790472076No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACCTGTCTCTTA2070.10156966845108709No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC16350.038.5300145
GCCGTCT2250.036.9979845
ACATAGT250.002101082136.006872
GCACGTG250.00210361535.9980359
ACGTGTG250.00210361535.99803511
ATGCCGT19300.032.6407144
ATCTCGT19600.032.25589437
CGAATCT19600.032.25589434
CATTCCG19600.032.14110629
CGAGACC19600.032.14110623
CTCGTAT19700.032.0921639
CGTATGC19750.032.01091441
CACGAGA19750.032.01091421
AATCTCG19750.032.01091436
ATTCCGA19800.031.9300830
AGACCAT19850.031.84964825
TCGTATG19850.031.84964840
CCACGAG19850.031.84964820
CCGAATC19750.031.78307733
GAATCTC19900.031.76962535
Produced by FastQC (version 0.11.2)