FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0120_pd_47.35100000046114.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0120_pd_47.35100000046114.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences395081
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGTC274476.947182982730124RNA PCR Primer, Index 46 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGT38060.9633467567410228No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAAC31790.8046451234050739No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA15340.3882748094694506No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCA15320.38776858416375376No Hit
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACC14830.375366064174182No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAACGC12500.31639081606050407No Hit
CCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCAC11680.295635578526935No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCC11010.27867703078609196No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAG10980.27791769282754675No Hit
GCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGT10800.27336166507627546No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA9360.23691344306610543No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA9010.22805450021641133No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCG7560.19135316555339285No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTC7540.19084694024769605No Hit
GCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATA6880.17414150515970142No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6750.17085104067267218No Hit
TATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGC6480.1640169990457653No Hit
GGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAA6450.1632576610872201No Hit
GAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAAC6340.16047342190588765No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACA6170.1561705068074648No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6150.15566428150176798No Hit
TATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA6010.15212070436189035No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTTT5840.1478177892634675No Hit
AAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGT5690.14402109947074143No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTT5650.14300864885934783No Hit
GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5630.14250242355365103No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA5540.1402244096780154No Hit
GTACGGGGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAGTCAGA5500.1392119590666218No Hit
CCACTGCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGT5420.13718705784383456No Hit
CTTCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGTTGATACCA5290.13389659335680532No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCACA5220.1321248047868665No Hit
GCTTCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGTTGATACC5220.1321248047868665No Hit
CACAGCATGTGCATTTTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCCT5190.1313654668283213No Hit
ACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGG5060.12807500234129204No Hit
CAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACG4870.12326586193717239No Hit
GTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGG4840.12250652397862716No Hit
CATATTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATCCTGTCTGAAT4730.11972228479729473No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGTG4550.11516625704602347No Hit
CATGCTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATCCTGTCTGAAT4530.11466003174032667No Hit
TTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGTCT4510.11415380643462986RNA PCR Primer, Index 18 (95% over 21bp)
CTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATAC4470.11314135582323624No Hit
GTATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA4190.10605420154348096No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTC4140.10478863827923894No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGATG4090.10352307501499693No Hit
GTATCAACGCAGAGTACGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA3990.1009919484865129No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCAT3990.1009919484865129No Hit
TCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGTTGATACCACT3970.10048572318081608No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC30300.043.14332245
ATGCCGT33850.038.6186944
GTATAAC356.22729E-638.580981
ATAACGC356.238126E-638.571213
TATGCCG34900.037.4568143
CTCGTAT34850.037.44598839
GTATGCC35000.037.41407842
CGTATGC34950.037.40322541
ATCTCGT34800.037.3704837
TCGTATG34900.037.3278740
CCGAATC34900.037.3278733
CGAATCT34800.037.3058334
CGAGACC35150.037.25441423
CATTCCG35000.037.15693729
GACCATT35200.037.07365426
CACGAGA35350.037.04363621
AGACCAT35400.036.99131825
AATCTCG35200.036.94581236
TCTCGTA35300.036.8411538
GAGACCA35550.036.8352324
Produced by FastQC (version 0.11.2)