FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0120_pd_45.351000000460ec.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0120_pd_45.351000000460ec.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37804
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGTC580815.363453602793356RNA PCR Primer, Index 46 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGT14573.8540895143371072No Hit
TTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGTCT3981.0527986456459633RNA PCR Primer, Index 18 (95% over 21bp)
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACA2680.7089196910379854No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTT1580.4179451909850809No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCG1080.2856840545973971No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAAC750.19839170458152577No Hit
ATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGTCTTC630.16664903184848165RNA PCR Primer, Index 46 (95% over 24bp)
CATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTTT610.1613585863929743No Hit
TATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGTCTT580.15342291820971327RNA PCR Primer, Index 46 (95% over 23bp)
TCTTTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCG550.14548725002645224No Hit
AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC500.13226113638768383No Hit
AAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCCA460.12168024547666914No Hit
CATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATC450.11903502274891546No Hit
GGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCA440.11638980002116178No Hit
AAAAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACCTGTCTCTT400.10580890911014706No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCA380.10051846365463973No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTCT850.039.70471645
TGCCGTC7450.039.2605945
ATGCCGT9400.031.11610844
ATCTCGT9650.030.54314437
AATCTCG9550.030.39177936
GAATCTC9500.030.314935
CGAATCT9500.030.07806434
TCTCGTA9800.030.0756538
ATCATTA602.2897257E-730.0388531
AATCCAT602.316574E-729.9991212
GAATCCA602.316574E-729.9991211
CCATAGG602.316574E-729.9991215
TCGTATG9900.029.77185240
CTCGTAT9900.029.77185239
GTATGCC10000.029.6991342
TATGCCG10000.029.6991343
CGTATGC9950.029.62224841
CCGAATC9650.029.61052933
GACCATT9900.029.54458626
ACCATTC9850.029.4661427
Produced by FastQC (version 0.11.2)