FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0120_pd_43.3510000004609c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0120_pd_43.3510000004609c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences128628
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGTC6012546.74332182728488RNA PCR Primer, Index 46 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGT105538.204279006126193No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCG55624.324097397145256No Hit
TTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGTCT2280.17725534098330067RNA PCR Primer, Index 18 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC63650.044.50510845
GCCGTCT650.041.5384645
ATGCCGT74950.037.79519744
TATGCCG80950.034.9938243
CTCGTAT80600.034.9224639
TCTCGTA80500.034.9099438
GTATGCC81150.034.87985242
ATCTCGT80550.034.86033637
CGTATGC81100.034.81812741
AATCTCG80350.034.80709536
TCGTATG80950.034.79925540
CCGAATC80750.034.71826633
GAATCTC80650.034.70551735
CGAATCT80650.034.70551734
GAGACCA81900.034.6703324
GACCATT81450.034.66850726
AGACCAT81650.034.6662625
GCCCACG81800.034.657718
CCCACGA81800.034.657719
CATTCCG81350.034.65580729
Produced by FastQC (version 0.11.2)