FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0120_pd_42.35100000046072.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0120_pd_42.35100000046072.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences116082
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGTC2761523.78921796660981RNA PCR Primer, Index 46 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGT114749.884392067676298No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCG11530.9932633827811375No Hit
TTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGTCT4190.36095174101066485RNA PCR Primer, Index 18 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC32300.043.18885445
GCCGTCT1350.036.66666445
ATGCCGT44050.031.66855844
CGTATGC45550.030.5762941
TATGCCG45650.030.55859843
CTCGTAT45550.030.52689439
TCGTATG45550.030.52689440
TCTCGTA45500.030.5109938
ATCTCGT45550.030.47749937
GTATGCC45700.030.4759342
AATCTCG45500.030.4615436
CCGAATC45450.030.44554333
TCCGAAT45400.030.37995532
CGAATCT45550.030.37870634
CACGAGA45850.030.37622521
CGAGACC45850.030.37622523
ACGAGAC45850.030.37622522
TTCCGAA45350.030.36383631
ATTCCGA45350.030.36383630
GACCATT45500.030.36263826
Produced by FastQC (version 0.11.2)