Basic Statistics
Measure | Value |
---|---|
Filename | H23LNBCXX_l01n01_bir_0120_pd_41.35100000046065.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 141684 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 44 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CTTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGTC | 34668 | 24.468535614465996 | RNA PCR Primer, Index 46 (95% over 21bp) |
TCTTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGT | 12927 | 9.123824849665453 | No Hit |
CTCTTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCG | 1465 | 1.0339911352022813 | No Hit |
TTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGTCT | 809 | 0.5709889613506113 | RNA PCR Primer, Index 18 (95% over 21bp) |
ATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGTCTTC | 183 | 0.1291606674006945 | RNA PCR Primer, Index 46 (95% over 24bp) |
TCTTTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCG | 146 | 0.10304621552186556 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
TGCCGTC | 3800 | 0.0 | 43.21894 | 45 |
ATGCCGT | 5095 | 0.0 | 32.233948 | 44 |
ATTCCGA | 5265 | 0.0 | 31.278616 | 30 |
CATTCCG | 5280 | 0.0 | 31.232365 | 29 |
ACGAGAC | 5300 | 0.0 | 31.199402 | 22 |
TTCCGAA | 5265 | 0.0 | 31.193153 | 31 |
TATGCCG | 5265 | 0.0 | 31.193153 | 43 |
GACCATT | 5310 | 0.0 | 31.183018 | 26 |
TCCGAGC | 5285 | 0.0 | 31.16025 | 13 |
CACGAGA | 5300 | 0.0 | 31.156954 | 21 |
CCGAGCC | 5300 | 0.0 | 31.156954 | 14 |
GAGACCA | 5330 | 0.0 | 31.150425 | 24 |
CTCGTAT | 5265 | 0.0 | 31.150423 | 39 |
TCTCGTA | 5265 | 0.0 | 31.150423 | 38 |
CCCACGA | 5290 | 0.0 | 31.130795 | 19 |
AGACCAT | 5335 | 0.0 | 31.12123 | 25 |
AATCTCG | 5270 | 0.0 | 31.12087 | 36 |
AGCCCAC | 5300 | 0.0 | 31.114506 | 17 |
CCACGAG | 5300 | 0.0 | 31.114506 | 20 |
CGAGACC | 5315 | 0.0 | 31.111353 | 23 |