FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0120_pd_41.35100000046065.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0120_pd_41.35100000046065.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences141684
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGTC3466824.468535614465996RNA PCR Primer, Index 46 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGT129279.123824849665453No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCG14651.0339911352022813No Hit
TTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGTCT8090.5709889613506113RNA PCR Primer, Index 18 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGTCTTC1830.1291606674006945RNA PCR Primer, Index 46 (95% over 24bp)
TCTTTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCG1460.10304621552186556No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC38000.043.2189445
ATGCCGT50950.032.23394844
ATTCCGA52650.031.27861630
CATTCCG52800.031.23236529
ACGAGAC53000.031.19940222
TTCCGAA52650.031.19315331
TATGCCG52650.031.19315343
GACCATT53100.031.18301826
TCCGAGC52850.031.1602513
CACGAGA53000.031.15695421
CCGAGCC53000.031.15695414
GAGACCA53300.031.15042524
CTCGTAT52650.031.15042339
TCTCGTA52650.031.15042338
CCCACGA52900.031.13079519
AGACCAT53350.031.1212325
AATCTCG52700.031.1208736
AGCCCAC53000.031.11450617
CCACGAG53000.031.11450620
CGAGACC53150.031.11135323