FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0120_pd_37.35100000045fd4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0120_pd_37.35100000045fd4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences68825
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTC32864.7744278968398115No Hit
TCTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGT9091.3207410098074828No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACA2740.3981111514711224No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAAC2170.3152924082818743No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTT2160.31383944787504536No Hit
TTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTCT1970.2862332001452961Illumina Paired End PCR Primer 2 (95% over 21bp)
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACCTGTCTCT1230.1787141300399564No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA1030.14965492190337815No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAACGC990.1438430802760625No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCA850.12350163458045768No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA730.10606610969851071No Hit
AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC720.1046131492916818No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCG700.10170722847802396No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC4200.042.31390845
CCAAGTA250.00209522535.9936039
GCCGTCT250.00209522535.99360345
TGTTTCC352.7840747E-432.16052230
CGACCTA5650.031.87591431
CTGCGAC5650.031.87591428
TGCGACC5650.031.87591429
ATCTCGT5750.031.69002337
TATCTCG5750.031.69002336
GACTGCG5550.031.63899826
CGTATGC5700.031.57333841
GTATGCC5700.031.57333842
TATGCCG5650.031.45458843
TCGTATG5800.031.4168340
CCTATCT5800.031.4168334
CACGAGA5450.031.37057521
GCGACCT5750.031.3215530
GACCTAT5750.031.3215532
CGAGACT5400.031.2444523
ATGCCGT5700.031.1786744
Produced by FastQC (version 0.11.2)