FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0120_pd_35.35100000045f91.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0120_pd_35.35100000045f91.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences135450
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTC7101452.4282022886674No Hit
TCTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGT1617411.940937615356221No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCG69855.1568844592100405No Hit
TTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTCT2780.2052417866371355Illumina Paired End PCR Primer 2 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC73800.044.51219645
GCCGTCT750.039.00000445
CCCGTAT356.2075724E-638.57142639
CTATCCC356.2075724E-638.57142635
ATGCCGT90000.036.544
CCTATCC458.624811E-735.00000434
ACCTATC97300.033.78468733
GCGACCT97500.033.76153630
TCCGAGC97850.033.75574513
GTATGCC97200.033.7542
TATCCCG401.5504647E-533.7536
AGCCCAC97750.033.74424417
GCCCACG97700.033.73848318
CCCACGA97700.033.73848319
TGCGACC97500.033.7384629
TATGCCG97300.033.73843843
GAGCCCA97850.033.73275416
CGAGCCC97800.033.72699415
CGACCTA97600.033.72694431
CCGAGCC97750.033.72122614
Produced by FastQC (version 0.11.2)