FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0120_pd_34.35100000045f84.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0120_pd_34.35100000045f84.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences138834
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTC2174515.662589855510896No Hit
TCTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGT46043.3161905585087226No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCG7150.5150035293948169No Hit
TTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTCT3400.2448967832087241Illumina Paired End PCR Primer 2 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCAGGA302.152634E-644.9971168
ATCGTTA207.01406E-444.99711616
TGCCGTC22700.043.41131645
ACAACCC250.002097690536.010675
ATAGTAC250.002097690536.010673
CATCGTT250.002101405835.99769615
ATGCCGT27950.035.17663644
CTGCGAC28550.034.20096228
GCCCACG28750.034.1978118
AGCCCAC28750.034.1978117
TATGCCG28750.034.1978143
TCCGAGC28500.034.18202213
GAGCCCA28700.034.17899716
ACGAGAC28900.034.1760122
ACTGCGA28650.034.16011427
CGACCTA28650.034.16011431
CGAGACT28850.034.15725723
TGCGACC28600.034.1411729
CCCACGA28800.034.1384419
CGAGCCC28550.034.12215815
Produced by FastQC (version 0.11.2)