FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0120_pd_33.35100000045f6a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0120_pd_33.35100000045f6a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences176691
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTC111706.321770774968731No Hit
TCTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGT23901.352643881125807No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCG4010.22694987294202873No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAAC3880.21959239576435696No Hit
TCATTAACCTATGGATTCAGTTAATGATAGCTGTCTCTTATACACATCTCC3330.18846460770497647No Hit
TTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTCT2160.1222473131059307Illumina Paired End PCR Primer 2 (95% over 21bp)
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCA2070.11715367505985025No Hit
CTATCATTAACTGAATCCATAGGTTAATGACTGTCTCTTATACACATCTCC2060.11658771527695243No Hit
CACAGCATGTGCATTTTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCCT2050.11602175549405458No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTTT1990.11262599679666763No Hit
CATGCTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATCCTGTCTGAAT1780.10074084135581327No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC12200.043.33934845
ATGCCGT14300.037.13216444
TATGCCG14500.036.6243
CTCGTAT14650.036.3986339
CGTATGC14600.036.3691841
TCGTATG14650.036.2450540
CACGAGA14800.036.1817521
CCACGAG14800.036.1817520
CTGCGAC14750.036.15186328
GACTGCG14750.036.15186326
ATCTCGT14800.036.02972437
TATCTCG14800.036.02972436
TGCGACC14800.036.02972429
GCGACCT14750.035.99932530
GTATGCC14750.035.99932542
CTTGTTA250.002102489835.999329
CGTGAGG250.002102489835.9993216
CCGTGAG250.002102489835.9993215
TACGATC250.002102489835.9993242
ACTGCGA14850.035.90841327
Produced by FastQC (version 0.11.2)