FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0120_pd_30.35100000045f0d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0120_pd_30.35100000045f0d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences300382
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCGTC99733.320105732034543No Hit
TCTTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCGT22630.7533740370594776No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAAC13920.46340992469588727No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACCTGTCTCT12870.4284544346864992No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTT7950.2646629957853666No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCA7140.2376973320638387No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGCTGTCTCTTATACACATCT6250.2080683929130241No Hit
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACC6060.20174311376846815No Hit
TCATTAACCTATGGATTCAGTTAATGATAGCTGTCTCTTATACACATCTCC5660.18842673662203463No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAACGC5580.18576346119274792No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACCTGTCT5340.17777363490488776No Hit
CCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCAC5120.17044962747434933No Hit
CTATCATTAACTGAATCCATAGGTTAATGACTGTCTCTTATACACATCTCC5050.16811926147372347No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACA4840.16112816347184586No Hit
AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC4830.160795254043185No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAG4820.1604623446145242No Hit
GCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGT4760.15846488804255915No Hit
CACTATCATTAACTGAATCCATAGGTTAATGACTGTCTCTTATACACATCT4330.14414978261014308No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA3980.13249795260701372No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACCTGTCTCTTA3810.12683849231977948No Hit
GTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCAC3730.12417521689049277No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGACTGTCTCTTATAC3370.11219047745870257No Hit
GTGTTTCGACACACTATCATTAACTGAATCCATAGGTTAATGACTGTCTCT3350.11152465860138092No Hit
AACCCAGTGTGTTTCGACACACTATCATTAACTGAATCCATAGGTTAATGA3100.10320192288485995No Hit
GCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATA3050.10153737574155576No Hit
TATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGC3050.10153737574155576No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTCT502.1827873E-1144.9966745
TGCCGTC11150.042.77710345
ACGGGAC301.1384102E-437.4972273
CGCGGCA250.002101960436.00932733
ATGCCGT13350.035.55916644
TATGCCG13800.034.39962843
CGTATGC13700.034.32227741
CCTTGCA13750.034.20886228
ATCTCGT13750.034.1974737
CGAGACC13900.034.15783723
CACGAGA13950.034.03540421
CCACGAG13950.034.03540420
CTTGCAG13850.033.9618729
ACCTTGC13900.033.8340727
GTATGCC13900.033.82843442
TCGTATG13900.033.66657640
GACCTTG14050.033.63300326
TTGCAGA14000.033.5979930
GAGACCT14200.033.59465824
GAATCTC14250.033.47678435
Produced by FastQC (version 0.11.2)