FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0120_pd_29.35100000045ed5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0120_pd_29.35100000045ed5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences86164
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCGTC71108.251706048929949No Hit
TCTTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCGT18892.19233090385776No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACA6370.7392878696439348No Hit
TTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCGTCT4820.5593983566222552RNA PCR Primer, Index 40 (95% over 21bp)
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTT3500.4062021261779862No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAAC2760.32031939092892625No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCG1590.1845318230351423No Hit
AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC1520.17640778051158254No Hit
AAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCCA1390.16132027296782878No Hit
GGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCA1310.1520356529409034No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAACGC1170.13578756789378393No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCA1120.12998468037695557No Hit
CATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTTT1080.12534237036349288No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA1070.12418179286012719No Hit
CATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATC960.11141544032310477No Hit
CCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCAC930.10793370781300776No Hit
AAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCACGA910.1056125528062764No Hit
GTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCAC870.1009702427928137No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC9500.039.5526345
GCCGTCT1150.037.17391245
AAGCCCA250.002096330735.99999616
AACCGGG1350.035.033
GCGAACC1350.035.030
GAACCGG1350.035.032
CCGGGGG1300.034.61538335
GGCGAAC1300.034.61538329
CGAACCG1400.033.75000431
ACCGGGG1350.033.33333234
GAGGCGA1350.033.33333227
ATAGGTT1500.033.017
AATGAGG1450.032.58620524
ATGAGGC1450.032.58620525
TAATGAG1450.032.58620523
ATGCCGT11600.032.3922444
GAACTGA1400.032.1428641
TGAAACA1400.032.1428645
TGAGGCG1400.032.1428626
CATAGGT1550.031.93548616
Produced by FastQC (version 0.11.2)