FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0120_pd_28.35100000045ebb.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0120_pd_28.35100000045ebb.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences290405
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCGTC4833916.64537456311014No Hit
TCTTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCGT86112.9651693324839448No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCG13040.44902808147242645No Hit
TTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCGTCT9610.33091716740414245RNA PCR Primer, Index 40 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCGTCTTC2920.10054923296775194RNA PCR Primer, Index 40 (100% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC52200.043.48888445
ATGCCGT60500.037.4482644
TATGCCG61750.036.61733243
TCTCGTA61350.036.5993738
GTATGCC61800.036.58770442
CGTATGC61750.036.58089441
ATCTCGT61450.036.5764237
CGAGCCC62000.036.5422615
ACGAGAC62250.036.5400722
AATCTCG61400.036.5329236
CTCGTAT61550.036.51699439
GCCCACG62150.036.4902618
GAGCCCA62150.036.4902616
TCGTATG61800.036.47848540
CACGAGA62300.036.47463221
CCACGAG62250.036.46778520
TCCGAGC62200.036.4609313
GACCTTG62300.036.4385226
CCCACGA62250.036.43164419
CCGAGCC62250.036.43164414
Produced by FastQC (version 0.11.2)