FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0120_pd_27.35100000045e92.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0120_pd_27.35100000045e92.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences287868
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCGTC120144.173440604721609No Hit
TCTTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCGT40881.4200953214667835No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAAC9000.3126432948434699No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACA8490.2949268414690066No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTT6000.20842886322897997No Hit
AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC5480.1903650284158017No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCG5110.17751191518334794No Hit
GTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCAC4910.17056428640904858No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCA4110.14277377131185126No Hit
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACC4060.14103686411827643No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACCTGTCTCT3770.1309628023955424No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAACGC3600.12505731793738797No Hit
GGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCA3370.11706754484694373No Hit
AAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCCA3340.11602540053079884No Hit
GCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGT2880.10004585434991037No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC11750.044.23267745
TCGCACT301.1380043E-437.4988426
ATGCCGT15800.032.8945544
GTGGCCA352.8156716E-432.1418659
CGCACTT352.8156716E-432.14186527
AAGTGCG352.8156716E-432.1418657
GCACTTC352.8156716E-432.14186528
TATGCCG16350.031.78800843
CGTATGC16350.031.65039641
TCGTATG16300.031.60945340
CTCGTAT16350.031.51278739
AATCTCG16350.031.37517536
ATCTCGT16450.031.3212237
GTATGCC16600.031.30927542
CCTTGCA16500.031.0899528
CACGAGA16800.031.07046921
TCTCGTA16600.031.03819838
GAGACCT16800.030.93654424
CGAGACC16750.030.89456723
GACCTTG16700.030.8523426
Produced by FastQC (version 0.11.2)