FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0120_pd_26.35100000045e78.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0120_pd_26.35100000045e78.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences141254
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCGTC116918.276579778271765No Hit
TCTTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCGT27901.9751653050533082No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCG4150.2937969898197573No Hit
TTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCGTCT1730.12247440780438076RNA PCR Primer, Index 40 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC12300.044.2606345
GCAAGGT250.00210265635.9937715
ATGCCGT15450.035.23661844
CGTATGC15600.034.753641
TATGCCG15750.034.56544543
GTATGCC15750.034.56544542
TCGTATG15650.034.49882540
CCTTGCA15800.034.32583228
ATCTCGT15800.034.31368337
CCCACGA15800.034.31368319
GAATCTC15750.034.2919235
AATCTCG15750.034.2797836
CACGAGA15900.034.2514821
GCCCACG15800.034.17130318
ACGAGAC15950.034.14410822
GACCTTG15950.034.14410826
ACCTTGC15900.034.10994327
TCTCGTA15900.034.0978738
CCACGAG15900.034.0978720
CGAGACC16000.034.0374123
Produced by FastQC (version 0.11.2)