FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0120_pd_25.35100000045e5f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0120_pd_25.35100000045e5f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences141757
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCGTC3352823.651742065647554No Hit
TCTTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCGT1632411.51548071700163No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCG22791.607680749451526No Hit
TTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCGTCT6410.4521822555499905RNA PCR Primer, Index 40 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATTTA207.012354E-445.011
TGCCGTC37200.043.85080745
GCCGTCT900.042.545
CCGATTT250.002100896336.010
GCAACTG250.002100896336.034
ACACCAT250.002100896336.06
TCTAGAC250.002100896336.03
ACACTTC300.005132225330.00000226
CTGGGAA300.005132225330.00000228
ATTTGAG300.005132225330.00000230
ATGCCGT54250.029.98617444
ATCTCGT56750.028.62555137
AATCTCG56650.028.59664736
TCGTATG56750.028.58590340
CGTATGC56850.028.575241
TATGCCG56950.028.5645343
GTATGCC56950.028.5645342
TCTCGTA56750.028.54625738
CTCGTAT56900.028.51054439
GCCCACG56950.028.44600518
Produced by FastQC (version 0.11.2)