H23LNBCXX_l01n01_bir_0120_pd_21.35100000045de3.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	H23LNBCXX_l01n01_bir_0120_pd_21.35100000045de3.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	35412
Sequences flagged as poor quality	0
Sequence length	51
%GC	42

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTC	5189	14.653224895515645	No Hit
TCTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGT	1222	3.450807635829662	No Hit
TTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTCT	297	0.838698746187733	RNA PCR Primer, Index 27 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTCTTC	120	0.3388681802778719	TruSeq Adapter, Index 27 (95% over 22bp)
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACA	119	0.3360442787755563	No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAAC	107	0.3021574607477691	No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTT	101	0.28521405173387554	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCG	86	0.24285552919914152	No Hit
TATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTCTT	67	0.18920140065514515	RNA PCR Primer, Index 27 (100% over 21bp)
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAACGC	66	0.18637749915282956	No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCG	63	0.17790579464588274	No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA	59	0.16661018863662036	No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA	57	0.16096238563198917	No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCA	47	0.13272337060883316	No Hit
AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC	41	0.11577996159493956	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAAACAC	20	6.9409393E-4	45.0	39
TGCCGTC	755	0.0	40.5298	45
ATGCCGT	905	0.0	33.563534	44
CACGAGA	895	0.0	33.435753	21
ATCTCGT	875	0.0	33.428574	37
ACGAGAC	890	0.0	33.37079	22
TATCTCG	870	0.0	33.36207	36
CGAGACT	885	0.0	33.305084	23
CGTATGC	900	0.0	33.25	41
TCGTATG	880	0.0	33.238636	40
CTCGTAT	880	0.0	33.238636	39
CTATCTC	880	0.0	33.238636	35
TATGCCG	915	0.0	33.19672	43
TCTCGTA	875	0.0	33.17143	38
CCCACGA	890	0.0	33.117977	19
CCACGAG	905	0.0	33.066296	20
GATCCTA	905	0.0	33.066296	31
CCTATCT	885	0.0	33.050846	34
TCCTATC	885	0.0	33.050846	33
AGACTAG	885	0.0	33.050846	25