FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0120_pd_19.35100000045da0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0120_pd_19.35100000045da0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences180224
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTC4835826.832164417613637No Hit
TCTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGT109896.097412109375No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCG29341.6279740767045456No Hit
TTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTCT4770.26467063210227276RNA PCR Primer, Index 27 (95% over 21bp)
AAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCCA2300.12761896306818182No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACA2020.11208274147727272No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTCT1050.045.00000445
AGTACTA207.0174446E-445.05
TGCCGTC52850.044.10595745
ATGCCGT64550.036.07668744
TATGCCG66950.034.682643
CTATCTC66750.034.51685335
ATCTCGT66900.034.5067337
GATCCTA66800.034.4910231
ATCCTAT66700.034.4752632
TCCTATC66700.034.4752633
GTATGCC67300.034.46879642
TATCTCG67050.034.4630936
CGTATGC67250.034.46096441
CCTATCT66750.034.4494434
TCTCGTA66950.034.4473538
CTCGTAT67100.034.4374139
TCGTATG67200.034.3861640
CACGAGA67900.034.36303321
AGATCCT67050.034.36241530
GACTAGA67600.034.34911326
Produced by FastQC (version 0.11.2)