FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0120_pd_16.35100000045d36.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0120_pd_16.35100000045d36.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23452
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTC469420.015350503155382TruSeq Adapter, Index 12 (95% over 21bp)
TTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTCT4011.7098754903632953TruSeq Adapter, Index 12 (95% over 22bp)
TCTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGT2841.2109841378134063No Hit
ATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTCTTC2040.8698618454716016TruSeq Adapter, Index 12 (95% over 24bp)
TATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTCTT770.3283302063789869RNA PCR Primer, Index 12 (95% over 23bp)
CTCTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCG710.3027460344533516No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTTC302.0797088E-644.98720645
TGCCGTC6400.036.20064545
ATGCCGT6700.034.5797244
TCCGAGC6700.034.5797213
CGAGCCC6800.034.4019815
CCGAGCC6800.034.4019814
CGAGACT6750.034.32357423
CTCCGAG6750.034.32357412
CTTATAC6900.034.302541
GCCCACG6700.034.2439918
CCCACGA6700.034.2439919
ACGAGAC6700.034.2439922
GTATGCC6800.034.07119442
TTGCAAT6800.034.07119432
ATCTCCG6800.034.07119410
TCGTATG6800.034.07119440
TCTCCGA6800.034.07119411
AGACTAC6800.034.07119425
TACTTGC6800.034.07119429
GAGACTA6800.034.07119424
Produced by FastQC (version 0.11.2)