FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0120_pd_10.35100000045c87.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0120_pd_10.35100000045c87.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences88405
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTC1135412.843164979356372TruSeq Adapter, Index 12 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGT15581.7623437588371698No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCG2670.3020191165658051No Hit
TTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTCT1780.20134607771053672TruSeq Adapter, Index 12 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTCTTG207.0014363E-444.99547227
TTCGTTC207.0014363E-444.99547224
TAGCTCC207.0014363E-444.99547241
TGCCGTC12300.044.2638445
ATGCCGT14100.038.6131444
CGTATGC14350.037.6268841
TCGTATG14350.037.6268840
ATCTCGT14300.037.60111237
TCTCGTA14300.037.60111238
TATGCCG14500.037.5479543
TCACCAA301.13208946E-437.4962320
TTTCGTC301.13208946E-437.4962331
TCGTCAT301.13208946E-437.4962333
CTCGTAT14350.037.470139
AATCTCG14350.037.470136
CCCACGA14600.037.2907719
CCACGAG14600.037.2907720
GACTACT14600.037.2907726
GCCCACG14550.037.26429418
AGCCCAC14550.037.26429417
Produced by FastQC (version 0.11.2)