FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0120_pd_1.35100000045b52.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0120_pd_1.35100000045b52.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences265441
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGTC39481.487336168866151RNA PCR Primer, Index 27 (95% over 21bp)
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAAC14200.5349588044047453No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCC11280.42495319110461455No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA7250.2731303754883383No Hit
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACC6910.2603215027068162No Hit
GTACGGGGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAGTCAGA6890.2595680396020208No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTC6830.2573076502876345No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCA6640.25014975079207813No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTTT5770.21737410573347749No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAA5290.1992909912183875No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCACA5090.19175636017043335No Hit
CCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCAC5070.19100289706563794No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAACGC4870.18346826601768376No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATAA4830.18196133980809295No Hit
GCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGT4530.17065939323616172No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGATG4370.16463168839779838No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA4350.16387822529300297No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAG4330.16312476218820754No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA4090.15408320493066258No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4030.1518228156162763No Hit
CATGCTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATCCTGTCTGAAT3900.1469253054351061No Hit
CACAGCATGTGCATTTTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCCT3890.1465485738827084No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGTG3800.14315798991112902No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCCGG3730.14052086904434508No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCAT3660.1378837481775611No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGTGTCCCGCC3580.13486989575837946No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3540.13336296954878862No Hit
CATATTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATCCTGTCTGAAT3530.13298623799639092No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATCACCTTACC3490.13147931178680008No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCAT3460.13034911712960695No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTC3450.12997238557720925No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA3420.12884219092001611No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCCGGTT3380.1273352647104253No Hit
TATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGC3350.12620507005323217No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCA3290.12394468073884592No Hit
GTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTC3270.12319121763405051No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTT3240.12206102297685738No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGA3180.11980063366247114No Hit
CACAATATGTGCATTTTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCCT3160.11904717055767572No Hit
GCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATA3160.11904717055767572No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATA3120.1175402443480849No Hit
TATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA3070.11565658658609634No Hit
TCTTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGT3030.11414966037650552No Hit
GTTCATATCACCTTACCGACGCTTATCGCAGATTAGCACGTCCTTCATCGC3030.11414966037650552No Hit
CATATCACCTTACCGACGCTTATCGCAGATTAGCACGTCCTTCATCGCCTC2970.11188927106211925No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTCTGCGGG2910.10962888174773301No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2850.10736849243334677No Hit
GGTTATAACGGTTCATATCACCTTACCGACGCTTATCGCAGATTAGCACGT2790.10510810311896053No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2780.10473137156656281No Hit
GGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAA2760.1039779084617674No Hit
GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2760.1039779084617674No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTTATAACCGG2750.10360117690936968No Hit
GAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAAC2680.10096405604258574No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACACACTGATT2670.10058732449018802No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC4100.041.70487245
CGTATGC4400.038.8613641
ATGCCGT4400.038.8613644
TATCTCG4450.038.42471336
GTATGCC4450.038.42471342
TCGTATG4450.038.42471340
TTGCTAT4350.038.2736232
GAATTGC4350.038.2736229
GACGAAT4350.038.2736226
ATCTCGT4500.037.99777237
CTCGTAT4500.037.99777239
TATGCCG4500.037.99777243
AGACGAA4500.037.99777225
GCTATCT4450.037.91912534
TGCTATC4450.037.91912533
CGAATTG4400.037.8386928
ACGAATT4400.037.8386927
CACGAGA4500.037.497821
GAGACGA4500.037.497824
CGAGACG4500.037.497823
Produced by FastQC (version 0.11.2)