FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0113_pc_96.35100000045b45.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0113_pc_96.35100000045b45.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36423
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCGACGGCATCTCGTATGCCGTC891024.46256486286138No Hit
ATACACATCTCCGAGCCCACGAGACTCGACGGCATCTCGTATGCCGTCTTC7542.070120528237652RNA PCR Primer, Index 34 (95% over 23bp)
TCTTATACACATCTCCGAGCCCACGAGACTCGACGGCATCTCGTATGCCGT6001.6473107651758505RNA PCR Primer, Index 41 (95% over 21bp)
TTATACACATCTCCGAGCCCACGAGACTCGACGGCATCTCGTATGCCGTCT3560.9774043873376712RNA PCR Primer, Index 41 (95% over 23bp)
TATACACATCTCCGAGCCCACGAGACTCGACGGCATCTCGTATGCCGTCTT1540.4228097630618016RNA PCR Primer, Index 34 (95% over 22bp)
CTCTTATACACATCTCCGAGCCCACGAGACTCGACGGCATCTCGTATGCCG1390.38162699393240535No Hit
CTATACACATCTCCGAGCCCACGAGACTCGACGGCATCTCGTATGCCGTCT430.11805727150426927RNA PCR Primer, Index 41 (95% over 23bp)
ACACATCTCCGAGCCCACGAGACTCGACGGCATCTCGTATGCCGTCTTCTG420.11531175356230954RNA PCR Primer, Index 34 (96% over 25bp)

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTTAA206.9435465E-445.01
TGCCGTC10850.039.1935545
ATGCCGT11600.036.6594844
GCATCTC11450.036.55021735
CTCGTAT11550.036.42857439
GTATGCC11650.036.30901342
ACTCGAC11650.036.30901327
CGTATGC11650.036.30901341
TCGTATG11650.036.30901340
GACTCGA11650.036.30901326
CATCTCG11600.036.2715536
CCACGAG11750.036.1914920
CACGAGA11750.036.1914921
CCCACGA11750.036.1914919
TCCGAGC11750.036.1914913
ATCTCCG11700.036.15384710
TATGCCG11700.036.15384743
TCTCGTA11650.036.1158838
ACGAGAC11850.036.07594722
AGCCCAC11800.036.03813617
Produced by FastQC (version 0.11.2)