FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0113_pc_94.35100000045b01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0113_pc_94.35100000045b01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences264927
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCGACGGCATCTCGTATGCCGTC111394.204554462172599No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA16810.6345144134044473No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14800.5586444567748852No Hit
GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13070.4933434493275506No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA11680.4408761658872067No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA11590.4374790036500621No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11060.41747349269798845No Hit
GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10640.4016200689246472No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8470.31971071276238355No Hit
TCTTATACACATCTCCGAGCCCACGAGACTCGACGGCATCTCGTATGCCGT8340.3148037006420637RNA PCR Primer, Index 41 (95% over 21bp)
GAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7970.30083758922269155No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA7790.2940432647484024No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7690.290268640040464No Hit
TATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA7330.2766799910918857No Hit
GTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7280.27479267873791646No Hit
GTATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA6090.22987464471344934No Hit
GAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA5470.2064719715242312No Hit
GTACGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA5240.19779033469597285No Hit
GTGTAGGATAGGTGGGAGGCTTTGAAGCGTGGACGCTAGTTCACGTGGAGC4850.18306929833501304No Hit
GGTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA4810.1815594484518377No Hit
GTACGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4430.1672158745616717No Hit
ATACACATCTCCGAGCCCACGAGACTCGACGGCATCTCGTATGCCGTCTTC4330.1634412498537333RNA PCR Primer, Index 34 (95% over 23bp)
ATCCTACACAGATCGGTTCAAAGTCCAATGCAAAGCTACAGTAAAGGTTCA3950.14909767596356732No Hit
TTATACACATCTCCGAGCCCACGAGACTCGACGGCATCTCGTATGCCGTCT3940.14872021349277348RNA PCR Primer, Index 41 (95% over 23bp)
TATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAA3860.14570051372642276No Hit
ATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA3800.1434357389016597No Hit
GTATCAACGCAGAGTACGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA3600.13588648948578286No Hit
GTATCAACGCAGAGTACGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAA3450.13022455242387526No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3410.1287147025406999No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG3350.12644992771593686No Hit
ACCATGCACTGTCCCCGACCCGGATTCACGGGCCAAGGTTAGAACCTCAAA3160.11927814077085386No Hit
GTACGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA3060.11550351606291545No Hit
GAGTACGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA2820.10644441676386325No Hit
GGTATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAA2790.10531202935148173No Hit
GAGTACGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA2780.10493456688068789No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG2720.10266979205592484No Hit
GTGCATGGTAGGCAGTTTGACTGGGGCGGTCTCCTCCCAAAGTGTAACGGA2720.10266979205592484No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATTAG207.020108E-445.0043451
TGCCGTC14150.040.0669845
ATTAGAT301.1381701E-437.496543
ATGCCGT15300.037.05540544
AGACTCG15550.036.61124825
GGCATCT15400.036.52949534
CACGAGA15650.036.52109521
ATCTCGT15550.036.4596637
GACGGCA15500.036.43931
CGTATGC15700.036.39791541
GTATGCC15700.036.39791542
TATGCCG15800.036.3099443
CCACGAG15750.036.28921520
CGGCATC15600.036.20541433
ACGGCAT15600.036.20541432
ACTCGAC15750.036.14634327
TAGTCCA250.00210319236.00347517
CTAAAGA250.002105142235.9966772
GATTAGA250.002105142235.9966772
CTCGACG15750.035.86060328
Produced by FastQC (version 0.11.2)