FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0113_pc_92.35100000045acc.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0113_pc_92.35100000045acc.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences722552
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCGACGGCATCTCGTATGCCGTC79831.104833977346959No Hit
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTCG10530.14573345586200026No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCCCAATCGGGCGGTAAATTC7780.10767391135862886No Hit
TCTTATACACATCTCCGAGCCCACGAGACTCGACGGCATCTCGTATGCCGT7490.1036603593928188RNA PCR Primer, Index 41 (95% over 21bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGAGGGG250.002106448636.000521
ACGGCAT10600.035.8731432
TATGCCG10750.035.78871543
ATGCCGT10800.035.62302844
CGTATGC10850.035.45886641
TCGTATG10850.035.25150340
TCGACGG11050.035.0231129
CTCGTAT11000.034.97533439
GTATGCC11100.034.86293442
CCCACGA11150.034.50720619
GGCATCT11100.034.4575534
CTCGACG11300.034.4473828
ACTCGAC11350.034.2956327
GCCCACG11400.033.94783818
TCTCGTA11350.033.69857838
CGAGACT11450.033.60308523
CATCTCG11250.033.5981436
GACTCGA11550.033.50695826
CACGAGA11750.033.1281221
GACGGCA11550.033.11734431
Produced by FastQC (version 0.11.2)