FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0113_pc_91.35100000045aa3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0113_pc_91.35100000045aa3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29942
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCGACGGCATCTCGTATGCCGTC817327.296105804555477No Hit
TCTTATACACATCTCCGAGCCCACGAGACTCGACGGCATCTCGTATGCCGT5721.9103600293901544RNA PCR Primer, Index 41 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACTCGACGGCATCTCGTATGCCGTCTTC3751.2524213479393496RNA PCR Primer, Index 34 (95% over 23bp)
TTATACACATCTCCGAGCCCACGAGACTCGACGGCATCTCGTATGCCGTCT2730.9117627412998464RNA PCR Primer, Index 41 (95% over 23bp)
CTCTTATACACATCTCCGAGCCCACGAGACTCGACGGCATCTCGTATGCCG1350.45087168525816573No Hit
TATACACATCTCCGAGCCCACGAGACTCGACGGCATCTCGTATGCCGTCTT1110.37071671899004743RNA PCR Primer, Index 34 (95% over 22bp)
CTATACACATCTCCGAGCCCACGAGACTCGACGGCATCTCGTATGCCGTCT560.1870282546256095RNA PCR Primer, Index 41 (95% over 23bp)
TCTTTATACACATCTCCGAGCCCACGAGACTCGACGGCATCTCGTATGCCG300.10019370783514794No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTTC253.805116E-544.98996745
TGCCGTC9200.040.5887745
ATGCCGT10000.037.34167544
TATGCCG10250.036.430943
CGTATGC10250.036.430941
TCGTATG10250.036.430940
CTCGTAT10150.036.34657739
GTATGCC10300.036.2540542
TCTCGTA10250.036.21143738
CATCTCG10150.036.1249536
GCATCTC10100.036.08106235
AGACTCG10350.036.07890725
GAGACTC10350.036.07890724
CTCCGAG10350.036.07890712
ATCTCGT10250.035.99197437
ACGGCAT10250.035.99197432
CGAGCCC10400.035.90545715
CCGAGCC10400.035.90545714
CGAGACT10400.035.90545723
GGCATCT10150.035.90332434
Produced by FastQC (version 0.11.2)